PMID: 7529335Feb 1, 1995Paper

Oligomeric rearrangement of tick-borne encephalitis virus envelope proteins induced by an acidic pH

Journal of Virology
Steven L AllisonFranz X Heinz

Abstract

The flavivirus envelope protein E undergoes irreversible conformational changes at a mildly acidic pH which are believed to be necessary for membrane fusion in endosomes. In this study we used a combination of chemical cross-linking and sedimentation analysis to show that the envelope proteins of the flavivirus tick-borne encephalitis virus also change their oligomeric structure when exposed to a mildly acidic environment. Under neutral or slightly alkaline conditions, protein E on the surface of native virions exists as a homodimer which can be isolated by solubilization with the nonionic detergent Triton X-100. Solubilization with the same detergent after pretreatment at an acidic pH, however, yielded homotrimers rather than homodimers, suggesting that exposure to an acidic pH had induced a simultaneous weakening of dimeric contacts and a strengthening of trimeric ones. The pH threshold for the dimer-to-trimer transition was found to be 6.5. Because the pH dependence of this transition parallels that of previously observed changes in the conformation and hydrophobicity of protein E and that of virus-induced membrane fusion, it appears likely that the mechanism of fusion with endosomal membranes involves a specific rearrangeme...Continue Reading

References

Jan 1, 1991·Archives of Virology·M F VorovitchL B Elbert
Aug 1, 1990·The Journal of General Virology·V B Randolph, V Stollar
Jan 1, 1990·Annual Review of Microbiology·T J ChambersC M Rice
Jan 1, 1990·Annual Review of Physiology·J M White
Jan 1, 1989·Advances in Virus Research·M Marsh, A Helenius
Jan 1, 1986·The Journal of General Virology·S W Gollins, J S Porterfield
Sep 1, 1985·The Journal of General Virology·S W Gollins, J S Porterfield
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Apr 1, 1984·The Journal of General Virology·M W Brandriss, J J Schlesinger
Feb 1, 1980·The Journal of General Virology·F X Heinz, C Kunz
Dec 1, 1993·Journal of Virology·J JustmanM Kielian

❮ Previous
Next ❯

Citations

Feb 14, 2004·Journal of Virology·Poonsook KeelapangNopporn Sittisombut
Dec 17, 2005·Nature Reviews. Microbiology·Margaret Kielian, Félix A Rey
Sep 16, 2006·Journal of Virology·Karin StiasnyFranz X Heinz
May 10, 2008·PLoS Pathogens·Steevenson NelsonTheodore C Pierson
Oct 22, 2008·The Journal of Cell Biology·Richard FritzFranz X Heinz
Mar 25, 2011·Journal of Virology·Mathilda SjöbergHenrik Garoff
Oct 1, 2011·The Journal of General Virology·Kentaro YoshiiIkuo Takashima
Jul 31, 2013·Quarterly Reviews of Biophysics·Michael G Rossmann
Feb 11, 2015·Viral Immunology·Kleber Juvenal Silva FariasBenedito Antônio Lopes da Fonseca
Jan 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ge LiRichard Y Zhao
Mar 1, 2017·The Journal of Microbiology·Sang-Im Yun, Young-Min Lee
Mar 22, 2002·Journal of Virology·Karin StiasnyFranz X Heinz
Jan 19, 2002·Annals of the New York Academy of Sciences·V DeubelP Desprès
Mar 22, 2002·Journal of Virology·Shu-Fen WuYi-Ling Lin
Dec 21, 2004·Nature Reviews. Microbiology·Suchetana MukhopadhyayMichael G Rossmann
Nov 27, 2004·Journal of Virology·Wayne D Crill, Gwong-Jen J Chang
Jan 28, 2006·Journal of Virology·Donna M TscherneCharles M Rice
Jan 23, 2004·Nature·Yorgo ModisStephen C Harrison
Apr 14, 2010·PLoS Pathogens·Aaron G SchmidtStephen C Harrison
Jun 20, 2008·Journal of Virology·Wolfgang FischlChristian W Mandl
Jan 23, 2008·Biochemical Society Transactions·Daniela S MuellerAlan E Mark
May 8, 2009·The Journal of General Virology·K L MansfieldT Solomon
May 13, 2008·Expert Reviews in Molecular Medicine·Theodore C Pierson, Michael S Diamond

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.