PMID: 11342001May 9, 2001Paper

Oligomerization of Escherichia coli enterotoxin b through its C-terminal hydrophobic alpha-helix

Biochimica Et Biophysica Acta
Vincent LabrieJ D Dubreuil

Abstract

Using a chemical cross-linker and gel electrophoresis or a dot blot overlay assay, we studied protein-protein interaction of STb toxin, a 48-residue amphiphilic polypeptide causing intestinal disorders. For the first time, we report on the oligomerization property of STb. This enterotoxin forms hexamers and heptamers in a temperature-independent fashion in presence or absence of its receptor (sulfatide) anchored in a 50-nm liposome or as a free molecule. Full STb structure integrity is necessary for its oligomerization as this process is not observed under reducing conditions in the presence of beta-mercaptoethanol. STb treatment with tetramethylurea (TMU) and different detergents prevented oligomerization. Site-directed mutagenesis decreasing overall STb hydrophobicity in the hydrophobic alpha-helix resulted in the incapacity to form oligomers. Taken together, these data suggest that the C-terminal hydrophobic alpha-helix corresponds to the domain of STb-STb inter-binding where hydrophobic interaction is involved.

References

Jul 21, 1988·Biochimica Et Biophysica Acta·I R MellorM S Sansom
Aug 1, 1993·Protein Expression and Purification·C E HandlJ D Dubreuil
Sep 1, 1995·Protein Science : a Publication of the Protein Society·M SukumarL M Gierasch
Feb 27, 1997·Nature·C PetosaR C Liddington

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Citations

Nov 2, 2001·Toxicon : Official Journal of the International Society on Toxinology·Hans-Erick BeausoleilJ Daniel Dubreuil
Feb 20, 2007·Infection and Immunity·Carina GonçalvesJ Daniel Dubreuil
Nov 25, 2003·Protein Science : a Publication of the Protein Society·Matthew J Gage, Anne Skaja Robinson
Jun 21, 2008·Journal of Medical Microbiology·Christine TaillonJ Daniel Dubreuil
Jul 10, 2012·Microbial Pathogenesis·H Claudia Syed, J Daniel Dubreuil
Dec 14, 2011·Toxicon : Official Journal of the International Society on Toxinology·Christine TaillonJ Daniel Dubreuil
Feb 19, 2009·FEMS Immunology and Medical Microbiology·Carina Gonçalves, J Daniel Dubreuil
Jan 6, 2011·FEMS Immunology and Medical Microbiology·Marie-Astrid AlbertJ Daniel Dubreuil
Apr 7, 2010·Critical Reviews in Microbiology·J Daniel Dubreuil
Oct 19, 2007·Journal of Biomolecular Screening·Felipe De Almeida DiasAngela Hampshire Lopes
Oct 14, 2016·EcoSal Plus·J Daniel DubreuilDieter M Schifferli

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