Oligonucleotide analysis by nanoparticle-assisted laser desorption/ionization mass spectrometry

The Analyst
Shu TairaYuko Ichiyanagi

Abstract

We analyzed oligonucleotides by nanoparticle-assisted laser desorption/ionization (nano-PALDI) mass spectrometry (MS). To this end, we prepared several kinds of nanoparticles (Cr-, Fe-, Mn-, Co-based) and optimized the nano-PALDI MS method to analyze the oligonucleotides. Iron oxide nanoparticles with diammonium hydrogen citrate were found to serve as an effective ionization-assisting reagent in MS. The mass spectra showed both [M - H](-) and [M + xMe(2+)- H](-) (Me: transition metal) peaks. The number of metal-adducted ion signals depended on the length of the oligonucleotide. This phenomenon was only observed using bivalent metal core nanoparticles, not with any other valency metal core nanoparticles. Our pilot study demonstrated that iron oxide nanoparticles could easily ionize samples such as chemical drugs and peptides as well as oligonucleotides without the aid of an oligonucleotide-specific chemical matrix (e.g., 3-hydroxypicolinic acid) used in conventional MS methods. These results suggested that iron-based nanoparticles may serve as the assisting material of ionization for genes and other biomolecules.

References

Apr 22, 1970·Journal of the American Chemical Society·S J ShawJ A McCloskey
Nov 1, 1996·Journal of Mass Spectrometry : JMS·K K Murray
Feb 1, 2006·Expert Review of Proteomics·Andrew M Dattelbaum, Srinivas Iyer
Jun 9, 2006·Environmental Science and Pollution Research International·Changbo ZhangNansheng Deng
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Koji IkegamiMitsutoshi Setou
Sep 20, 2007·Biotechnology and Bioengineering·Shu TairaMasato Kodaka
Feb 8, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Alex Xiu-Cheng FanXiao Yan Zhong
Apr 11, 2008·Nucleic Acids Research·Jennifer V DitlevsonPhilip C Hanawalt
Apr 22, 2008·Methods in Molecular Biology·Chunming Ding
Jun 19, 2009·Nature·Sung Wook ChiRobert B Darnell
Jun 27, 2009·Cell Biology International·A H M Khurshid AlamToshifumi Tsukahara
Sep 12, 2009·Nature·Jeff Tollefson
May 1, 2010·Journal of the American Society for Mass Spectrometry·Cheng-Kang ChiangHuan-Tsung Chang
Jul 14, 2010·Molecular Cell·Chanseok ShinDavid P Bartel
Aug 4, 2010·Chemistry : a European Journal·Hideya KawasakiRyuichi Arakawa
Jan 26, 2011·Analytical Chemistry·Shu TairaYuko Ichiyanagi
Jul 14, 2009·Science and Technology of Advanced Materials·Shu TairaYuko Ichiyanagi

❮ Previous
Next ❯

Citations

Jan 1, 2013·Journal of Personalized Medicine·Patrick A GladdingTodd T Schlegel
Sep 15, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuichi IwakiRyuichi Arakawa
Jun 3, 2021·The Analyst·Yuri YamadaNorihiro Mizoshita

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.