Oligonucleotide-based fluorogenic sensor for simultaneous detection of heavy metal ions

Biosensors & Bioelectronics
Changlong HaoChuanlai Xu

Abstract

In this study, we report a new fluorogenic sensor based on fluorescence resonance energy transfer (FRET) for detection of heavy metal ions in aqueous solution. The method showed the advantage of being simple, highly sensitive and selective, and rapid. The donor (CdTe QDs) and acceptor (TAMRA or Cy5) are brought into close proximity to one another due to Hg(2+) and Ag(+) form strong and stable T-Hg(2+)-T complexes and C-Ag(+)-C complexes, which quenches the fluorescent intensity of CdTe QDs and enables the energy transfer from donor to acceptor. This sensor showed high sensitivity and selectivity when only one kind of ion (Ag(+) or Hg(2+)) exists. Furthermore, the assay can also simultaneously detect Ag(+) and Hg(2+) in water media with the limit of detection (LOD) of 2.5 and 1.8 nM, separately, which satisfactorily meets the sensitivity demands of Environmental Protection Agency (EPA) and World Health Organization (WHO). This assay also exhibits excellent selectivity toward Ag(+) and Hg(2+). Therefore, this method is of great practical and theoretical importance for detecting heavy metal ions in aqueous solution.

References

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Citations

May 24, 2019·The Analyst·Xiaobin WangXinsheng Peng
Oct 31, 2014·The Analyst·Guichi Zhu, Chun-yang Zhang
Aug 31, 2012·Chemical Communications : Chem Comm·Mary Anitha ArugulaZhen He
Nov 26, 2013·Chemical Communications : Chem Comm·Guichi ZhuChun-yang Zhang
Nov 10, 2017·Chemical Society Reviews·Jacek L KolanowskiElizabeth J New
Oct 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Modi WangDik-Lung Ma
Jan 27, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Chunli SunFan Xia
Dec 15, 2020·Journal of Hazardous Materials·Harpreet SinghNeha Bhardwaj
Jun 19, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Li ZhangJian-Ding Qiu

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