PMID: 9447700Jul 1, 1997Paper

Oligonucleotide biological activity: relationship to the cell cycle and nuclear transport

Biology of the Cell
S Wu-PongJ Jimerson

Abstract

Previous studies suggest that oligodeoxynucleotide (ODN) cellular uptake is cell cycle-dependent which may have important implications in cancer cell targeting. To further our understanding of ODN transport and activity, this study examines the relationships between the cell cycle, ODN cellular uptake, intracellular transport, and activity. An antisense c-myc ODN 21-mer was used to study ODN cellular uptake in Rauscher erythroleukemia cells synchronized by either chemical methods or flow cytometry. ODN uptake was examined using subcellular fractionation and confocal fluorescence microscopy. Western blot analysis was used to measure ODN-mediated decreases in c-myc protein levels. Intracellular ODN distribution and extent of uptake was influenced by the phase of the cell cycle, but the mechanism of uptake was not. The relative activity of the antisense ODN was positively correlated to ODN distribution to the cytosol, but negatively correlated to total cellular uptake. Although ODN total cellular uptake is positively influenced by the cell cycle, retention of the ODN in the cytosol (presumably extra-vesicularly) appeared to be relevant in determining the activity of an antisense ODN. Novel methods to target cytosol-acting drugs to...Continue Reading

Citations

Dec 25, 2007·Oligonucleotides·Zhaoyang YeRam I Mahato
May 17, 2005·Nitric Oxide : Biology and Chemistry·Karsten HemmrichVictoria Kolb-Bachofen
Nov 22, 2005·Expert Opinion on Drug Delivery·Ram I MahatoRamareddy V Guntaka
Jul 29, 2003·Journal of Pharmaceutical Sciences·Melanie A Lysik, Susanna Wu-Pong
Mar 28, 2003·American Journal of Physiology. Cell Physiology·Karsten HemmrichVictoria Kolb-Bachofen

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