Oligonucleotide cleavage and rejoining by topoisomerase III from the hyperthermophilic archaeon Sulfolobus solfataricus: temperature dependence and strand annealing-promoted DNA religation

Molecular Microbiology
Liang Chen, Li Huang

Abstract

Topoisomerase III from the hyperthermophilic archaeon Sulfolobus solfataricus (Sso topo III) is optimally active in DNA relaxation at 75 degrees C. We report here that Sso topo III-catalysed DNA cleavage and religation differed significantly in temperature dependence: the enzyme was most active in cleaving ssDNA containing a cleavage site at 25-50 degrees C, but was efficient in rejoining the cleaved DNA strand only at higher temperatures (e.g. > or = 45 degrees C). The failure of Sso topo III to rejoin the cleaved DNA strand efficiently appeared to be responsible for the inability of the enzyme to relax negatively supercoiled DNA at low temperature (e.g. 25 degrees C). Intriguingly, Sso topo III facilitated DNA annealing although it showed higher affinity for ssDNA than for dsDNA. Religation of the DNA strand cleaved by Sso topo III was drastically enhanced when the DNA was allowed to anneal to a complementary non-cleaved oligonucleotide, presumably as a result of destabilization of the interaction between the enzyme and the cleaved strand through the formation of duplex DNA. A region in the non-cleaved strand corresponding to a sequence containing six bases on the 5' side and two bases on the 3' side of the cleavage site in t...Continue Reading

References

Nov 1, 1986·Molecular & General Genetics : MGG·N IrinoH Nakayama
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P O Brown, N R Cozzarelli
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·D B Wigley
Apr 12, 1996·Science·C E YuG D Schellenberg
May 1, 1996·FEMS Microbiology Reviews·P ForterreP Lopez-Garcia
May 13, 1999·Nature Genetics·S KitaoY Furuichi
Jul 3, 1999·Trends in Biochemical Sciences·J E Haber
Mar 29, 2000·The Journal of Biological Chemistry·L WuI D Hickson
Jan 1, 2001·The Journal of Biological Chemistry·W M FrickeS J Brill
Jun 8, 2001·Annual Review of Biochemistry·J J Champoux
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·Q ZhuR J DiGate
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·R J Bennett, J C Wang
Jun 4, 2002·Nature Reviews. Molecular Cell Biology·James C Wang
Aug 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·N H DekkerV Croquette
Jan 2, 2003·The Journal of Biological Chemistry·Pearl NurseKenneth J Marians

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Citations

May 7, 2011·Biochemistry·Zhongfeng ZuoMichael A Trakselis
Aug 12, 2014·Extremophiles : Life Under Extreme Conditions·Antonella VettoneMaria Ciaramella
Sep 27, 2014·International Journal of Molecular Sciences·Valeria VisoneMaria Ciaramella
Mar 26, 2011·Journal of Molecular Biology·Anna BizardMarc Nadal
Feb 13, 2007·Biochimie·Patrick ForterreMarie-Claude Serre
Dec 19, 2017·Nucleic Acids Research·Anna H BizardMarc Nadal

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