Oligonucleotide mixture analysis via electrospray and ion/ion reactions in a quadrupole ion trap

Analytical Chemistry
S A McLuckeyGregory B Hurst

Abstract

Electrospray ionization combined with ion/ion reactions in a quadrupole ion trap can be used for the direct analysis of oligonucleotide mixtures. Elements to the success of this approach include factors related to ionization, ion/ion reactions, and mass analysis. This paper deals with issues regarding the ion polarity combination, viz., positive oligonucleotides/negative charge-transfer agent versus negative oligonucleotides/positive charge-transfer agent. Anions derived from perfluorocarbons appear to be directly applicable to mixtures of positive ions derived from electrospray of oligonucleotides, in direct analogy with positive protein ions. Conditions for forming positive oligonucleotide ions devoid of adducts were more difficult to establish than for forming relatively clean negative oligonucleotide ions. A new approach for manipulating negative ion charge states in the ion trap is described and is based on use of the electric field of the positive charge-transfer agent for storage of high-mass negative ions formed during the ion/ion reaction period. Oxygen cations are shown to be acceptable for charge-state manipulation of mixed-base oligomers but induce fragmentation in polyadenylate homopolymers. Protonated isobutylene ...Continue Reading

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Citations

Mar 30, 2004·Journal of the American Society for Mass Spectrometry·Bart Van den DriesscheEddy L Esmans
Jan 18, 2005·Journal of the American Society for Mass Spectrometry·Yu XiaJames W Hager
May 26, 2005·Journal of the American Society for Mass Spectrometry·Paul A ChrismanScott A McLuckey
Sep 27, 2005·Journal of the American Society for Mass Spectrometry·Yu XiaScott A McLuckey
Dec 18, 2007·Journal of the American Society for Mass Spectrometry·Christopher K BarlowScott A McLuckey
Dec 18, 2007·Journal of the American Society for Mass Spectrometry·Yu Xia, Scott A McLuckey
Oct 12, 2010·Journal of the American Society for Mass Spectrometry·J Larry Campbell, J C Yves Le Blanc
Jun 12, 2002·Journal of the American Society for Mass Spectrometry·J Mitchell WellsScott A McLuckey
Sep 18, 2009·Analytical Chemistry·Scott A McLuckey, Teng-Yi Huang
Jul 20, 2010·Annual Review of Analytical Chemistry·Teng-Yi Huang, Scott A McLuckey
Nov 19, 2010·Bioanalysis·Hong-Wu Shen, Ai-Ming Yu
Jan 5, 2014·Journal of the American Society for Mass Spectrometry·Dan GuoWei Xu
Jan 7, 2009·Rapid Communications in Mass Spectrometry : RCM·Joshua F EmoryScott A McLuckey
Apr 15, 2015·Journal of the American Society for Mass Spectrometry·Dan GuoWei Xu
Jul 19, 2002·Journal of Mass Spectrometry : JMS
Feb 12, 2005·Mass Spectrometry Reviews·Sharon J Pitteri, Scott A McLuckey
Dec 22, 2012·Chemical Communications : Chem Comm·Boone M Prentice, Scott A McLuckey
Jan 25, 2011·Rapid Communications in Mass Spectrometry : RCM·Kerry M HassellScott A McLuckey
Mar 17, 2019·Journal of the American Society for Mass Spectrometry·Kenneth W LeeScott A McLuckey
Oct 1, 2021·Chemical Reviews·Eric LargyValérie Gabelica

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