PMID: 3768497Jun 1, 1986Paper

Oligonucleotides as probes for mammalian protamine mRNAs

Bioscience Reports
S A KrawetzG H Dixon

Abstract

Protamine-like sequences have been identified in poly A(+)mRNAs from mammalian testes by the use of a common, complementary oligonucleotide (GCAGCANCKPTANCKNGCCAT; predicted from the common N-terminal amino acid sequence, MARYRCC, seen in several mammalian P1 protamines [D.J. McKay, B.S. Renaux and G.H. Dixon, Bioscience Reports 5:383-391 (1985)]). This oligonucleotide was utilized to prepare species-specific, primer-extended transcripts for use as Northern blotting probes. Analysis of the mRNA primer-extended transcripts revealed a discrete and similar set of products common to both bull and rat testis mRNAs which were distinct from those obtained from human testis mRNA. Northern analysis of total poly A(+) mRNAs from the corresponding mammalian testis was consistent with these results and suggests that bull and rat protamine mRNAs are more closely related to each other than to human protamine mRNA.

References

Jan 1, 1976·Analytical Biochemistry·J M Bailey, N Davidson
Apr 14, 1976·Biochimica Et Biophysica Acta·W S KistlerR L Heinrikson
Jan 1, 1986·Journal of Biochemical and Biophysical Methods·S A KrawetzG H Dixon
May 1, 1985·Bioscience Reports·D J McKayG H Dixon
Nov 28, 1972·Biochimica Et Biophysica Acta·J P CoelinghA Ruhfus
Jul 1, 1984·Bioscience Reports·S A Krawetz, G H Dixon
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jan 1, 1984·Annual Review of Biochemistry·K ItakuraR B Wallace
May 1, 1983·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·S A KrawetzR A Anwar

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