Oligosaccharide and peptidoglycan of Ganoderma lucidum activate the immune response in human mononuclear cells

Journal of Agricultural and Food Chemistry
Chia-Che TsaiShih-Hsiung Wu

Abstract

The acid-hydrolyzed fragments of Ganoderma lucidum polysaccharides (GLPS) obtained by Smith degradation were separated by size-exclusion chromatography into two major water-soluble fractions: peptidoglycans (GLPS-SF1) and oligosaccharides (GLPS-SF2). Both fractions induced CD69 in human peripheral blood mononuclear cells (hPB-MNCs), and they displayed distinct immunomodulating properties. GLPS-SF1, with a molecular weight of around 20 kDa, were heterogeneous peptidoglycans composed of glucose/mannose (4:1) that exhibited biological activities with Th1 cytokines IL-12, IL-2, TNF-α, and IFN-γ in hPB-MNCs and stimulated macrophage cytokine expression via Toll-like receptor 4 (TLR4) signaling. For GLPS-SF2, with a molecular weight of around several kilodaltons, its sugar sequence was elucidated by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy as [-α-1,4-Glc-(β-1,4-GlcA)(3)-](n). This oligosaccharide displayed specific immune property with low monocyte induction, greatly stimulated cell activation and proliferation of NK and T cells. This oligosaccharide isolated from G. lucidum polysaccharides with internal glucuronic acids/glucose repeat unit in a 3:1 ratio may be responsible for the active stimulation o...Continue Reading

References

Jul 15, 1987·Carbohydrate Research·A K RayN Roy
May 1, 1994·Biochemical Society Transactions·P AlbersheimA Whitcombe
Nov 5, 1999·Archives of Pharmacal Research·J CheongW Park
Dec 10, 2002·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Raymond Chang
Aug 6, 2003·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Ming-Shi Shiao
Jun 8, 2004·Current Medicinal Chemistry·Benny K H Tan, J Vanitha
Oct 7, 2004·Bioorganic & Medicinal Chemistry·Hung-Sen ChenChi-Huey Wong
Nov 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hsien-Yeh HsuChi-Huey Wong
Mar 16, 2005·Analytical Biochemistry·Tatsuya MasukoYuan C Lee
Oct 19, 2005·Journal of Pharmacological Sciences·Zhi-Bin Lin
Jan 10, 2006·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Wing Keung ChanGodfrey Chi-Fung Chan
Aug 15, 2006·Phytochemistry·R Russell M Paterson
Sep 19, 2007·Biotechnology Annual Review·Bojana BohLin Zhi-Bin
Dec 17, 2008·Combinatorial Chemistry & High Throughput Screening·Chia-Che TsaiShui-Tein Chen
Oct 20, 2009·The Journal of Biological Chemistry·Tsui-Ling HsuChi-Huey Wong
Oct 30, 2009·Journal of Agricultural and Food Chemistry·Jianguo WangPeter C K Cheung
Jan 8, 2011·The American Journal of Chinese Medicine·Zengtao XuYitao Wang

❮ Previous
Next ❯

Citations

Nov 10, 2017·Journal of Asian Natural Products Research·Zhen TanJun-Gui Dai
Jul 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li-Zhi ChengYong-Xian Cheng
Apr 18, 2019·Journal of Agricultural and Food Chemistry·Yan-Li WuLi-Xia Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.