Oligosaccharide sequences in Quillaja saponins by electrospray ionization ion trap multiple-stage mass spectrometry

Journal of Mass Spectrometry : JMS
Susanna BrobergLennart Kenne

Abstract

Ten different samples with 13 previously identified saponin structures from Quillaja saponaria Molina were investigated by electrospray ionization ion trap multiple-stage mass spectrometry (ESI-ITMS(n)) in positive and negative ion modes. Both positive and negative ion mode MS(1)-MS(4) spectra were analyzed, showing that structural information on the two oligosaccharide parts in the saponin can be obtained from positive ion mode spectra whereas negative ion mode spectra mainly gave information on one of the oligosaccharide parts. Analysis of MS(1)-MS(4) spectra identified useful key fragment ions important for the structural elucidation of Quillaja saponins. A flowchart involving a stepwise procedure based on key fragments from MS(1)-MS(3) spectra was constructed for the identification of structural elements in the saponin. Peak intensity ratios in MS(3) spectra were found to be correlated with structural features of the investigated saponins and are therefore of value for the identification of terminal monosaccharide residues.

Citations

Apr 11, 2006·Journal of the American Society for Mass Spectrometry·Tobias MadlGerald N Rechberger
Jun 18, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Mingquan GuoZhiqiang Liu
Dec 17, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Johan BankeforsLennart Kenne
Mar 30, 2005·Rapid Communications in Mass Spectrometry : RCM·Fabrizio Dal PiazNunziatina De Tommasi
Jun 28, 2011·Journal of Mass Spectrometry : JMS·Johan BankeforsLennart Kenne
Jan 7, 2015·Rapid Communications in Mass Spectrometry : RCM·Sarina M DucksteinFlorian C Stintzing
May 20, 2015·Journal of Pharmaceutical and Biomedical Analysis·Yong-Gang XiaHai-Xue Kuang
Jul 17, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Katarzyna Mikołajczyk-BatorPiotr Kachlicki
Oct 29, 2004·Phytochemical Analysis : PCA

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