Oligosaccharides from depolymerized fucosylated glycosaminoglycan: Structures and minimum size for intrinsic factor Xase complex inhibition

The Journal of Biological Chemistry
Ronghua YinJinhua Zhao

Abstract

Fucosylated glycosaminoglycan (FG), a structurally complex glycosaminoglycan found up to now exclusively in sea cucumbers, has distinct anticoagulant properties, notably a strong inhibitory activity of intrinsic factor Xase complex (FXase). Knowledge of the FG structures could facilitate the development of a clinically effective intrinsic FXase inhibitor for anticoagulant drugs. Here, a new fucosylated glycosaminoglycan was obtained from the widely traded sea cucumber Bohadschia argus The precise structure was deduced as {→4)-[l-Fuc3S4S-α-(1→3)-]-d-GlcA-β-(1→3)-d-GalNAc4S6S-β-(1} through analysis of its chemical properties and homogeneous oligosaccharides purified from its β-eliminative depolymerized products. The B. argus FG with mostly 3,4-di-O-sulfated fucoses expands our knowledge on FG structural types. This β-elimination process, producing oligosaccharides with well-defined structures, is a powerful tool for analyzing the structure of complex FGs. Among these oligosaccharides, an octasaccharide displayed potent FXase inhibitory activity. Compared with oligosaccharides with various degrees of polymerization (3n and 3n - 1), our analyses reveal that the purified octasaccharide is the minimum structural unit responsible for ...Continue Reading

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Dec 9, 2014·Analytical Chemistry·Yu HuangJoseph Zaia
Jun 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Longyan ZhaoJinhua Zhao
Feb 19, 2017·International Journal of Biological Macromolecules·Jie LiuJinhua Zhao
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Feb 25, 2018·European Journal of Medicinal Chemistry·Feineng ShangJinhua Zhao

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Citations

Jun 5, 2020·Marine Drugs·Giulia VessellaEmiliano Bedini
Oct 18, 2020·Marine Drugs·Rohini Dwivedi, Vitor H Pomin
Sep 18, 2020·European Journal of Medicinal Chemistry·Huifang SunJinhua Zhao
Apr 23, 2021·Comprehensive Reviews in Food Science and Food Safety·Zhenjun ZhuHang Xiao
Mar 31, 2019·Carbohydrate Polymers·Gislaine C Dos Santos-FidencioDiogo R B Ducatti

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