Olive oil DNA fingerprinting by multiplex SNP genotyping on fluorescent microspheres

Journal of Agricultural and Food Chemistry
Despina P KalogianniPenelope C Ioannou

Abstract

Olive oil cultivar verification is of primary importance for the competitiveness of the product and the protection of consumers and producers from fraudulence. Single-nucleotide polymorphisms (SNPs) have emerged as excellent DNA markers for authenticity testing. This paper reports the first multiplex SNP genotyping assay for olive oil cultivar identification that is performed on a suspension of fluorescence-encoded microspheres. Up to 100 sets of microspheres, with unique "fluorescence signatures", are available. Allele discrimination was accomplished by primer extension reaction. The reaction products were captured via hybridization on the microspheres and analyzed, within seconds, by a flow cytometer. The "fluorescence signature" of each microsphere is assigned to a specific allele, whereas the signal from a reporter fluorophore denotes the presence of the allele. As a model, a panel of three SNPs was chosen that enabled identification of five common Greek olive cultivars (Adramytini, Chondrolia Chalkidikis, Kalamon, Koroneiki, and Valanolia).

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Citations

Mar 19, 2016·Journal of the Science of Food and Agriculture·Antonella PasqualoneFrancesco Caponio
May 28, 2019·Analytical and Bioanalytical Chemistry·Aikaterini GalaziouPenelope C Ioannou
Jan 24, 2019·Frontiers in Plant Science·Nicolò G M CultreraLuciana Baldoni
Mar 23, 2019·Journal of Agricultural and Food Chemistry·Karola BöhmeIgnacio Ortea

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