Omasal sampling technique for assessing fermentative digestion in the forestomach of dairy cows

Journal of Animal Science
P HuhtanenL D Satter

Abstract

A procedure allowing digesta sampling from the omasum via a ruminal cannula without repeated entry into the omasum was developed. The sampling system consisted of a device inserted into the omasum via the ruminal cannula, a tube connecting the device to the ruminal cannula, and a single compressor/vacuum pump. Eight cows given ad libitum access to a total mixed diet were used in a crossover design to evaluate the effects of the sampling system on digestive activity, animal performance, and animal behavior. Results indicated that the omasal sampling system has minimal effect on normal digestive and productive functions of high-producing dairy cows. Dry matter intake was reduced (24.0 vs 21.8 kg/d; P < .02) and seemed related more to the sampling procedures than to the device in the omasum. Observations of animal behavior indicated that cows with the sampling device were similar to control cows, although rumination and total chewing times were reduced slightly. The composition of digesta samples was biased toward an over-abundance of the liquid phase, but using a double-marker system to calculate digesta flow resulted in fairly small coefficients of variation for measurements of ruminal digestion variables. This technique may pro...Continue Reading

Citations

Mar 23, 2012·Animal : an International Journal of Animal Bioscience·S J KrizsanS Colombini
Jan 19, 1999·Journal of Dairy Science·J L FirkinsN R St-Pierre
Jul 8, 2003·The British Journal of Nutrition·Seppo AhvenjärviPekka Huhtanen
Nov 29, 2008·Journal of Dairy Science·G A BroderickV A Ishler
Apr 12, 2006·Journal of Dairy Science·S AhvenjärviP Huhtanen
Feb 1, 2008·Animal : an International Journal of Animal Bioscience·S I Borucki CastroR Berthiaume
Nov 21, 2017·Journal of Dairy Science·Charles G Schwab, Glen A Broderick
Apr 30, 2019·Journal of Dairy Science·A N HristovZ Yu
Dec 19, 2012·Journal of Dairy Science·P P RayK F Knowlton

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