Abstract
We reported that breast density (BD) was inversely correlated with the plasma level of DHA in postmenopausal obese, but not in nonobese, women given Lovaza (n-3FA). To identify protein biomarkers for the possible differential effect of n-3FA on BD between obese and nonobese women, an iTRAQ method was performed to analyze plasma from obese and lean women at each time point (baseline, 12 and 24-months, n = 10 per group); 173 proteins with >95% confidence (Unuses Score >1.3 and local false discovery rate estimation <5%) were identified. Comparative analysis between various groups identified several differentially expressed proteins (hemopexin precursor, vitamin D binding protein isoform 1 precursor [VDBP], fibronectin isoform 10 precursor [FN], and α-2 macroglobulin precursor [A2M]). Western blot analysis was performed to verify the differential expression of proteins in the iTRAQ study, and those found to be altered in a tumor protective fashion by an n-3FA rich diet in our previous preclinical study; gelsolin, VDBP, and FN were altered by n-3FA in a manner consistent with reduction in inflammation in obese women. To test the impact of our findings on breast cancer risk reduction by n-3FA, a posthoc analysis revealed that n-3FA a...Continue Reading
References
Jun 4, 2002·DNA and Cell Biology·Emanuela Tolosano, Fiorella Altruda
Jul 13, 2004·Trends in Biotechnology·Peter T Gomme, Joseph Bertolini
Jun 7, 2006·JAMA : the Journal of the American Medical Association·Victor G VogelUNKNOWN National Surgical Adjuvant Breast and Bowel Project (NSABP)
Nov 28, 2007·Annual Review of Pathology·Thea D Tlsty, Lisa M Coussens
Dec 17, 2008·Current Protein & Peptide Science·Robert BuckiPaul A Janmey
Mar 24, 2009·Metabolism: Clinical and Experimental·Stephen J WintersJames J Miller
Dec 29, 2010·Journal of Proteome Research·James D BortnerKaram El-Bayoumy
May 26, 2011·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·John A ShepherdSteven R Cummings
Aug 5, 2011·Cancer Prevention Research·Zongjian ZhuHenry J Thompson
Sep 22, 2011·Cancer Research·Carina SignoriAndrea Manni
Jun 2, 2012·Cancer Research·Weiqin JiangHenry J Thompson
Sep 18, 2012·Lipids in Health and Disease·Elena BurilloFernando Civeira
Oct 12, 2012·Nature Biotechnology·Matthew C ChambersParag Mallick
Jul 25, 2013·Cancer Prevention Research·Christine G SkibinskiKaram El-Bayoumy
Jun 22, 2014·Breast Cancer Research and Treatment·Susann E SchetterAndrea Manni
Aug 31, 2014·Cancer Research·Rosa Anna DeFilippisThea D Tlsty
Mar 5, 2015·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·Bashar A ZeidanRamsey I Cutress
Oct 7, 2015·Cancer Prevention Research·Carol J FabianStephen D Hursting
Dec 31, 2015·Cancer Prevention Research·Narinder SandhuAndrea Manni
Oct 12, 2016·JAMA : the Journal of the American Medical Association·Elizabeth D KantorEdward L Giovannucci
Nov 18, 2016·Oncoimmunology·Annelie AbrahamssonCharlotta Dabrosin
Oct 7, 2017·Scientific Reports·Claudia AgnoliVittorio Krogh
Mar 13, 2018·EBioMedicine·Trevor W StoneL Gail Darlington