Omega-3 Polyunsaturated Fatty Acids May Attenuate Streptozotocin-Induced Pancreatic β-Cell Death via Autophagy Activation in Fat1 Transgenic Mice

Endocrinology and Metabolism
Won Min HwangJae Gu Kang

Abstract

Inflammatory factors and β-cell dysfunction due to high-fat diets aggravate chronic diseases and their complications. However, omega-3 dietary fats have anti-inflammatory effects, and the involvement of autophagy in the etiology of diabetes has been reported. Therefore, we examined the protective effects of autophagy on diabetes using fat-1 transgenic mice with omega-3 self-synthesis capability. Streptozotocin (STZ) administration induced β-cell dysfunction in mice; blood glucose levels and water consumption were subsequently measured. Using hematoxylin and eosin (H&E) and Masson's trichrome staining, we quantitatively assessed STZ-induced changes in the number, mass, and fibrosis of pancreatic islets in fat-1 and control mice. We identified the microtubule-associated protein 1A/1B light chain 3-immunoreactive puncta in β-cells and quantified p62 levels in the pancreas of fat-1 and control mice. STZ-induced diabetic phenotypes, including hyperglycemia and polydipsia, were attenuated in fat-1 mice. Histological determination using H&E and Masson's trichrome staining revealed the protective effects of the fat-1 expression on cell death and the scarring of pancreatic islets after STZ injection. In the β-cells of control mice, auto...Continue Reading

References

Mar 5, 2002·Diabetes Care·Rob M van DamFrank B Hu
Feb 7, 2004·Nature·Jing X KangZhao B Kang
Jan 20, 2005·Molecular and Cellular Biology·Patricia BoyaGuido Kroemer
Jul 18, 2006·The American Journal of Clinical Nutrition·Philip C Calder
Aug 7, 2009·Autophagy·Piero Marchetti, Matilde Masini
Oct 12, 2010·Autophagy·Claudio D GonzalezJohn W Wiley

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Citations

Jul 19, 2016·Journal of Clinical Pharmacy and Therapeutics·H-S KimK-H Yoon
Apr 22, 2017·The Journal of Endocrinology·Alpana MathurPoonam Kakkar
Apr 5, 2018·Endocrinology·Michelle R Marasco, Amelia K Linnemann
Sep 27, 2018·Cells·Manon Moulis, Cecile Vindis
Feb 20, 2020·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Medha PriyadarshiniBrian T Layden

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Methods Mentioned

BETA
confocal microscopy
protein assay
electrophoresis

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