Omeprazole promotes gastric epithelial cell migration

The Journal of Pharmacy and Pharmacology
Ka-Man NgShou-Dong Lee

Abstract

Proton pump inhibitors (PPIs) are effective at preventing non-steroidal anti-inflammatory drug (NSAID)-induced gastric ulcers. They are also superior to histamine H(2)-receptor antagonists and misoprostol in treating NSAID-induced gastric ulcer healing. This study explored whether omeprazole, a PPI, can modulate ulcer healing through epithelial cell proliferation and/or cell migration using a rat normal gastric epithelial cell line (RGM-1). Flow cytometry was used to determine cell proliferation and an artificial wound model was used to measure cell migration. Western blot analysis was performed to evaluate the possible mechanisms of action. Omeprazole treatment (10(-8), 10(-6) and 10(-4)M) for 12 and 24 h did not promote cell proliferation. However, similar doses of the drug (10(-6) and 10(-4)M) incubated for 24-48 h significantly promoted the basal cell migration of gastric epithelial cells. Further, the higher concentration of omeprazole (10(-4)M) reversed the inhibitory action of indometacin (10(-5)) on cell migration. Western blot results showed that omeprazole did not increase cyclooxygenase-2 expression and did not activate signal transduction pathways, including extracellular signal-regulated kinase (ERK1/ERK2), P38 mit...Continue Reading

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Citations

Jul 29, 2011·Journal of Gastroenterology and Hepatology·Young Woo KimSang Geon Kim
Oct 20, 2009·Journal of the Chinese Medical Association : JCMA·Jiing-Chyuan LuoShou-Dong Lee
Feb 9, 2017·Canadian Journal of Physiology and Pharmacology·Maja KostrzewskaAndrzej Dabrowski
Aug 25, 2020·ACS Chemical Neuroscience·Rehab E Abo El GheitMarwa N Emam

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