Omics Analysis Reveals the Mechanism of Enhanced Recombinant Protein Production Under Simulated Microgravity

Frontiers in Bioengineering and Biotechnology
Jie HuangfuChun Li

Abstract

Simulated microgravity (SMG) is regarded as a suitable environment to produce recombinant proteins. This study showed that β-glucuronidase expressing Escherichia coli had higher productivity of recombinant protein and higher plasmid copy number under SMG compared with the normal gravity condition. The cellular changes were analyzed at both transcriptomic and proteomic levels. The upregulation of a group of ribosome/RNA polymerase genes and a cluster of genes involving energy metabolism at transcriptomic level stood out for improved production of recombinant protein under SMG. The protein folding modulators such as chaperones were upregulated at proteomic level, which could be a result of the increased activity of protein synthesis and can help recombinant protein production. Protein export was also strengthened, which was revealed at both transcriptomic and proteomic levels. The results demonstrated that SMG is a favorable environment for recombinant protein production arousing the upregulation of protein synthesis, protein folding, and protein export.

References

Nov 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·E Kaltschmidt, H G Wittmann
May 8, 2001·Science·J M OgleV Ramakrishnan
Oct 31, 2002·Applied and Environmental Microbiology·James W WilsonCheryl A Nickerson
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Cheryl A NickersonDuane L Pierson
Jun 26, 2004·Advances in Biochemical Engineering/biotechnology·Frank Hoffmann, Ursula Rinas
Nov 6, 2004·Nature Biotechnology·François Baneyx, Mirna Mujacic
Jan 18, 2005·Cell·Seyedtaghi TakyarHarry F Noller
Mar 17, 2005·Microgravity Science and Technology·Paul W BakerLaura G Leff
Sep 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·J W WilsonC A Nickerson
Jun 6, 2009·Nature Structural & Molecular Biology·F Ulrich Hartl, Manajit Hayer-Hartl
Oct 23, 2009·Applied Microbiology and Biotechnology·Jason A RosenzweigOlufisayo Jejelowo
Nov 19, 2009·Applied Biochemistry and Biotechnology·Liang XiangYuanDa Jiang
Dec 1, 2010·Journal of the American Chemical Society·Yanxiang ShiWilfred A van der Donk
Dec 3, 2014·Methods in Molecular Biology·Roger G Harrison, Miguel J Bagajewicz
Feb 17, 2015·Journal of Molecular Biology·Eugenia M ClericoLila M Gierasch
Oct 1, 2015·Trends in Biochemical Sciences·Manajit Hayer-HartlF Ulrich Hartl

❮ Previous
Next ❯

Citations

May 4, 2021·Frontiers in Microbiology·Xinyi ChenHu Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
protein folding
electrophoresis
RNA-seq
glycosylation
environmental stress
proteomics profiling

Software Mentioned

MASCOT Daemon
Data Analysis
Cytoscape
SFOER

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.