On an aerodynamic mechanism to enhance ion transmission and sensitivity of FAIMS for nano-electrospray ionization-mass spectrometry

Journal of the American Society for Mass Spectrometry
Satendra PrasadRandy W Purves

Abstract

Simulations show that significant ion losses occur within the commercial electrospray ionization-field asymmetric waveform ion mobility spectrometer (ESI-FAIMS) interface owing to an angular desolvation gas flow and because of the impact of the FAIMS carrier gas onto the inner rf (radio frequency) electrode. The angular desolvation gas flow diverts ions away from the entrance plate orifice while the carrier gas annihilates ions onto the inner rf electrode. A novel ESI-FAIMS interface is described that optimizes FAIMS gas flows resulting in large improvements in transmission. Simulations with the bromochloroacetate anion showed an improvement of ~9-fold to give ~70% overall transmission). Comparable transmission improvements were attained experimentally for six peptides (2+) in the range of m/z 404.2 to 653.4 at a chromatographic flow rate of 300 nL/min. Selected ion chromatograms (SIC) from nano-LC-FAIMS-MS analyses showed 71% (HLVDEPQNLIK, m/z 653.4, 2+) to 95% (LVNELTEFAK, m/z 582.3, 2+) of ion signal compared with ion signal in the SIC from LC-MS analysis. IGSEVYHNLK (580.3, 2+) showed 24% more ion signal compared with LC-MS and is explained by enhanced desolvation in FAIMS. A 3-10 times lower limits of quantitation (LOQ) (<...Continue Reading

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Citations

Feb 5, 2016·Journal of the American Society for Mass Spectrometry·Helen J Cooper
Mar 25, 2015·Journal of the American Society for Mass Spectrometry·Nicholas E Manicke, Michael Belford
Aug 20, 2016·Rapid Communications in Mass Spectrometry : RCM·Fang Yuan GaoQing He Zhang
Nov 1, 2016·Journal of the American Society for Mass Spectrometry·Andrew J Creese, Helen J Cooper
Oct 11, 2016·Journal of Proteome Research·Sibylle PfammatterPierre Thibault
Jan 18, 2017·Journal of the American Society for Mass Spectrometry·Randy W PurvesJean-Jacques Dunyach
Apr 7, 2018·Journal of the American Society for Mass Spectrometry·Sibylle PfammatterPierre Thibault
Jan 18, 2021·Proteomics·Joao A Paulo, Devin K Schweppe
Aug 18, 2018·Journal of the American Society for Mass Spectrometry·Ri WuT-W Dominic Chan
Nov 18, 2020·Journal of the American Society for Mass Spectrometry·Pratima PathakAlexandre A Shvartsburg
Jul 4, 2018·Analytical Chemistry·Pratima PathakAlexandre A Shvartsburg
Jul 4, 2018·Analytical Chemistry·Alexander S HebertJoshua J Coon
Jan 23, 2020·Analytical Chemistry·Rian L GriffithsHelen J Cooper
Oct 16, 2021·Expert Review of Proteomics·Xinyue LiuJoao A Paulo
Jan 7, 2022·Journal of Proteomics·Ching-Seng AngNicholas A Williamson

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