On and off membrane dynamics of the endoplasmic reticulum-golgi tethering factor p115 in vivo.

Molecular Biology of the Cell
Elizabeth BrandonElizabeth Sztul

Abstract

The mechanisms regulating membrane recruitment of the p115 tethering factor in vivo are unknown. Here, we describe cycling of p115 between membranes and cytosol and document the effects of Golgi matrix proteins, Rab1, and soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptors (SNAREs) on this process. Rapid membrane/cytosol exchange is shown by swift (t1/2 approximately 20 s) loss of Golgi-localized p115-green fluorescent protein (GFP) after repeated photobleaching of cell periphery and rapid (t1/2 approximately 13 s) fluorescence recovery after photobleaching Golgi-localized p115-GFP. p115 mutant missing the GM130/giantin binding site exhibits analogous fluorescence recovery after photobleaching (FRAP) (t1/2 approximately 13 s), suggesting that GM130 and giantin are not major determinants of p115 membrane dynamics. In contrast, p115-GFP exchanges more rapidly (t1/2 approximately 8 s) in cells expressing the inactive Rab1/N121I mutant, indicating that p115 cycling is influenced by Rab1. p115-GFP dynamics is also influenced by the assembly status of SNAREs. In cells expressing an ATPase-deficient NSF/E329Q mutant that inhibits SNARE complex disassembly, the cycling kinetics of p115-GFP are significan...Continue Reading

References

May 1, 1992·The Journal of Cell Biology·D W WilsonJ E Rothman
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J SalameroK E Howell
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·S K SappersteinM G Waters
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·M BarrosoE Sztul
Mar 25, 1993·Nature·T SöllnerJ E Rothman
Dec 1, 1995·The Journal of Cell Biology·N NakamuraG Warren
Mar 1, 1996·The Journal of Cell Biology·S K SappersteinM G Waters
Dec 1, 1996·Archives of Biochemistry and Biophysics·M R MejillanoR H Himes
Jan 7, 1998·The Journal of Cell Biology·C Barlowe
Apr 18, 1998·The Journal of Cell Biology·B SönnichsenG Warren
May 16, 1998·The Journal of Cell Biology·M AridorW E Balch
Jun 12, 1998·The Journal of Cell Biology·S M VanRheenenM G Waters
Dec 2, 1998·Journal of Biochemistry·T YamaguchiM Tagaya
Dec 22, 1999·The Journal of Cell Biology·C AlvarezE Sztul
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·D F SealsA Price
Oct 18, 2000·The Journal of Biological Chemistry·C AlvarezE Sztul
May 16, 2001·International Review of Cytology·S W WhiteheartE A Matveeva
Nov 14, 2001·The Journal of Cell Biology·T H WardJ Lippincott-Schwartz
May 10, 2002·Nature·John F PresleyJennifer Lippincott-Schwartz
Mar 27, 2003·Traffic·Ayano SatohGraham Warren

❮ Previous
Next ❯

Citations

May 23, 2012·Histochemistry and Cell Biology·Mónica TomásJosé A Martínez-Menárguez
Dec 14, 2011·Protoplasma·Nathanael P Cottam, Daniel Ungar
Jan 26, 2013·PloS One·Mayuko KoreishiAyano Satoh
Nov 6, 2009·FEBS Letters·Elizabeth Sztul, Vladimir Lupashin
Jan 30, 2016·Molecular Biology of the Cell·Ashley J RussoKenneth G Campellone
Jan 27, 2015·Biochemical Society Transactions·Saralin Davis, Susan Ferro-Novick
Apr 24, 2015·Molecular Biology of the Cell·Valentina MillarteHesso Farhan
Sep 21, 2012·Bioarchitecture·Robert GrabskiElizabeth Sztul
Mar 22, 2014·Cellular Logistics·Rose WillettVladimir Lupashin
Mar 4, 2010·Journal of Pharmacological Sciences·Nobuhiro Nakamura
Feb 23, 2018·Molecular Biology of the Cell·Leslie K ClimerVladimir V Lupashin
Oct 19, 2017·Translational Stroke Research·Dong YuanBingren Hu
Oct 14, 2006·The Journal of Biological Chemistry·Marvin BentleyJesse C Hay
Apr 7, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Haijun GongRussell Schwartz
Oct 21, 2011·Physiology·Tomasz Szul, Elizabeth Sztul
Jan 3, 2008·The Journal of Biological Chemistry·Aipo DiaoMartin Lowe

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.