On-Bead Two-Color (OBTC) Cell Screen for Direct Identification of Highly Selective Cell Surface Receptor Ligands.

Current Protocols in Chemical Biology
D Gomika Udugamasooriya, Thomas Kodadek

Abstract

Combinatorial library screens can identify a suitable ligand for a biological target of interest out of thousands or even millions of compounds, and can play a key role in the modern drug development process. While conventional high-throughput cell screens based on functional assays require expensive robotics, simple on-bead combinatorial assays for ligand binding to the target protein can be done far more cheaply. This article describes one such assay, developed using combinatorial peptoid libraries for targeting integral membrane receptors or other cell surface-exposed molecules. In addition to the reduced cost, a unique advantage of this assay is the direct identification of the most selective ligands for a cell surface receptor that is expressed in its natural environment.

References

Oct 18, 2002·Biopolymers·Olulanu H AinaKit S Lam
Nov 13, 2003·Journal of the American Chemical Society·Prasanna G AlluriThomas Kodadek
Nov 13, 2003·Chembiochem : a European Journal of Chemical Biology·Hernando J OlivosThomas Kodadek
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May 9, 2006·Journal of Combinatorial Chemistry·Margot G PaulickRonald N Zuckermann
Jun 1, 2007·Journal of the American Chemical Society·Hyun-Suk LimThomas Kodadek
Apr 5, 2008·Journal of the American Chemical Society·D Gomika UdugamasooriyaThomas Kodadek
Jul 26, 2008·Bioorganic & Medicinal Chemistry Letters·D Gomika UdugamasooriyaThomas Kodadek
Aug 13, 2008·Combinatorial Chemistry & High Throughput Screening·Lisa K Minor
Apr 9, 2009·Journal of the American Chemical Society·Levi S SimpsonThomas Kodadek
Nov 18, 2009·Biotechnology Journal·Jennifer A MaynardSang-Hyun Oh
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Aug 28, 2010·Journal of the American Chemical Society·Luis M De León-RodríguezA Dean Sherry
Nov 6, 2010·Assay and Drug Development Technologies·Christopher B BlackR Terry Dunlay

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Citations

Feb 24, 2016·Accounts of Chemical Research·Yan ShiJianfeng Cai
Oct 29, 2015·ACS Chemical Biology·Jaya M MatharageD Gomika Udugamasooriya
Dec 29, 2016·Biochemistry·Olapeju BolarinwaJianfeng Cai
Oct 26, 2013·Chemical Communications : Chem Comm·Haifan WuJianfeng Cai
Nov 5, 2016·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Lina Y HuJulie L Sutcliffe

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