On-chip amperometric measurement of quantal catecholamine release using transparent indium tin oxide electrodes

Analytical Chemistry
Xiuhua Sun, K D Gillis

Abstract

Carbon-fiber amperometry has been extensively used to monitor the time course of catecholamine release from cells as individual secretory granules discharge their contents during the process of quantal exocytosis, but microfabricated devices offer the promise of higher throughput. Here we report development of a microchip device that uses transparent indium tin oxide (ITO) electrodes to measure quantal exocytosis from cells in microfluidic channels. ITO films on a glass substrate were patterned as 20-mum-wide stripes using photolithography and wet etching and then coated with polylysine to facilitate cell adherence. Microfluidic channels (100 mum wide by 100 mum deep) were formed by molding poly(dimethylsiloxane) (PDMS) on photoresist and then reversibly sealing the PDMS slab to the ITO-glass substrate. Bovine adrenal chromaffin cells were loaded into the microfluidic channel and adhered to the ITO electrodes. Cells were stimulated to secrete by perfusing a depolarizing "high-K" solution while monitoring oxidation of catecholamines on the ITO electrode beneath the cell using amperometry. Amperometric spikes with charges ranging from 0.1 to 1.5 pC were recorded with a signal-to-noise ratio comparable to that of carbon-fiber elec...Continue Reading

References

Dec 11, 1991·Proceedings of the National Academy of Sciences of the United States of America·R M WightmanO H Viveros
Jan 18, 1967·Journal of the American Chemical Society·M D HawleyR N Adams
Sep 1, 1993·The Journal of Physiology·Z Zhou, E Neher
Oct 17, 1998·Science·M A BurnsD T Burke
Nov 21, 2000·Journal of Neuroscience Methods·F SeguraR Borges
Jun 29, 2001·Nature·S TakayamaG M Whitesides
Jul 16, 2002·Annual Review of Biomedical Engineering·David J BeebeGlenn M Walker
Jan 15, 2005·Analytical Chemistry·Vladimir Parpura
Apr 2, 2005·Analytical Chemistry·Makiko GotoTakehiko Kitamori
Dec 1, 1998·Analytical Chemistry·D C DuffyG M Whitesides

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Citations

Mar 13, 2008·Analytical and Bioanalytical Chemistry·Ning BaoChang Lu
Jun 4, 2010·Analytical and Bioanalytical Chemistry·Shencheng GeChristy L Haynes
Sep 29, 2009·Analytical Chemistry·Khajak BerberianManfred Lindau
Jul 1, 2008·Annual Review of Analytical Chemistry·Kelly L AdamsAndrew G Ewing
Sep 20, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jiehua LinShusheng Zhang
Jan 20, 2012·Biophysical Chemistry·Anne MeunierChristian Amatore
Oct 28, 2006·Cell Calcium·Martin OheimWalter Stühmer
Jun 19, 2012·Journal of Biomaterials and Nanobiotechnology·Kassandra KislerManfred Lindau
Jun 14, 2016·Analytical Methods : Advancing Methods and Applications·J YaoK D Gillis
Mar 25, 2015·The Analyst·Christian AmatoreFrédéric Lemaître
Jul 28, 2018·Chemistry, an Asian Journal·Sabine KussRichard G Compton
Sep 4, 2017·Pflügers Archiv : European journal of physiology·Kevin D GillisValentina Carabelli
Oct 15, 2018·FEBS Letters·Erdem Karatekin
Oct 20, 2007·Angewandte Chemie·Albert Schulte, Wolfgang Schuhmann
Aug 26, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Lisa MellanderAndrew G Ewing
Sep 11, 2017·Pflügers Archiv : European journal of physiology·Meng HuangManfred Lindau
Dec 22, 2009·Lab on a Chip·Gregory M Dittami, Richard D Rabbitt
Mar 15, 2011·Advanced Materials·Sang Yoon YangGeorge G Malliaras
May 27, 2021·Analytical Chemistry·Meng HuangManfred Lindau
Feb 24, 2007·Biophysical Chemistry·Christian AmatoreYann Verchier

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