On-chip, aptamer-based sandwich assay for detection of glycated hemoglobins via magnetic beads

Biosensors & Bioelectronics
Jinglun LiGwo-Bin Lee

Abstract

Diabetes can be diagnosed and monitored by measurement of the cutoff ratio between glycated hemoglobins (HbA1c) and total hemoglobin (Hb), which does not require a fasting blood sample and is less influenced by biological variations. In this study, we combined the advantages of the microfluidic system and the selected low-cost, stable and specific aptamers and developed an integrated, aptamer-based microfluidic system for automatic glycated hemoglobin measurements. The detection process of human whole blood can be totally automated in this integrated microfluidic system. According to the experimental results, when compared to conventional bench-top manual assays, reagent consumption was significantly reduced by 75%, and the analysis time was reduced from 3.5h to 30 min. Besides, the novelty in this research also lies in the simultaneously performed two parallel assays for detection of Hb and HbA1c in a single chip. Therefore, this sensitive and low-cost aptamer-based microfluidic system may become a promising tool for point-of -care diagnosis of diabetes.

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Citations

Mar 18, 2016·The Protein Journal·Maryam Tabarzad, Marzieh Jafari
Feb 26, 2020·Micromachines·Niazul I Khan, Edward Song
Apr 11, 2018·NPJ Digital Medicine·Jiandong WuFrancis Lin
Nov 17, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jingping HuZhiguang Zhou
Nov 15, 2018·Micromachines·Donny Nugraha MazaafriantoManabu Tokeshi
Apr 2, 2020·International Journal of Biological Macromolecules·Pradakshina SharmaJagriti Narang
Nov 27, 2021·Advanced Science·Yuntao LiuGuoqing Pan
May 17, 2018··Vyacheslav I. KochubeyVasiliy A. Slezhkin

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