On-chip detection of protein glycosylation based on energy transfer between nanoparticles

Biosensors & Bioelectronics
Young-Pil KimHak-Sung Kim

Abstract

We describe a chip-based method to detect protein glycosylation based on the energy transfer between quantum dots (QDs) and gold nanoparticles (AuNPs). Our assay system relies on modulations in the energy transfer between the nanoparticles on a surface. The photoluminescence (PL) of lectin-coated QDs (energy donor) immobilized on a glass slide is quenched by carbohydrate-coated AuNPs (energy acceptor), and the presence of the glycoprotein causes the increase of the PL of QDs. As a proof-of-concept, Concanavalin A-coated QDs (ConA-QDs) and dextran-coated AuNPs (Dex-AuNPs) were used to detect the mannosylated proteins. As a result, the PL intensity of QDs was found to be linearly correlated with the concentration and the number of glycan moiety of the glycoprotein. We anticipated that our simple assay system will find applications for the analysis of glycoproteins with high selectivity and sensitivity in a high-throughput manner.

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Citations

Sep 5, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hong Hanh TranNae Yoon Lee
May 9, 2012·Quarterly Reviews of Biophysics·Christiane Höppener, Lukas Novotny
Sep 1, 2012·ACS Nano·Mohamed Shehata DrazFanqing Frank Chen
Apr 17, 2010·Nanomedicine·Vinay J NagarajShalini Prasad
Sep 21, 2013·International Journal of Molecular Sciences·Jianhao WangPengju Jiang
Jan 29, 2014·International Journal of Molecular Sciences·Lin QiuPengju Jiang
Dec 26, 2015·Chemical Reviews·Martina DelbiancoPeter H Seeberger
Apr 11, 2012·Biotechnology and Bioengineering·Germarie Sánchez-PomalesRebecca A Zangmeister
Jun 4, 2015·Nanomedicine·Sona KrizkovaRene Kizek
Jun 28, 2011·Analytical Biochemistry·Xiaodong CaoSongqin Liu
Dec 7, 2018·Journal of Carbohydrate Chemistry·Crystal L O'NeilAlexei V Demchenko

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