On-chip electrophoresis in supported lipid bilayer membranes achieved using low potentials

Journal of the American Chemical Society
Jasper van WeerdPascal Jonkheijm

Abstract

A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25-1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.

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Citations

Feb 18, 2016·ACS Applied Materials & Interfaces·Tao ZhuYong Hu
Aug 15, 2014·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Sven O KrabbenborgJurriaan Huskens
Nov 29, 2016·Advanced Healthcare Materials·Gülistan Koçer, Pascal Jonkheijm
Apr 1, 2014·Chemical Society Reviews·Jenny BrinkmannPascal Jonkheijm
Apr 1, 2017·Chemical Communications : Chem Comm·P BaoS D Evans
Nov 18, 2015·Advanced Healthcare Materials·Jasper van WeerdPascal Jonkheijm
Jun 26, 2014·Angewandte Chemie·Sven O Krabbenborg, Jurriaan Huskens
May 26, 2020·Advanced Materials·Jieun KimJwa-Min Nam
Jun 11, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Toshinori MotegiKei Murakoshi
Dec 31, 2020·ACS Central Science·Nico J OvereemJurriaan Huskens
Jul 21, 2020·Journal of the American Chemical Society·Simou SunPaul S Cremer

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Methods Mentioned

BETA
electrophoresis
Fluorescence Recovery After Photobleaching
fluorescence microscopy
chip
chips

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