On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids

Nanoscale
Hendrik DeschoutKevin Braeckmans

Abstract

Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid. However, an unbound dye in the biofluid can cause high background intensity that strongly biases the fluorescence single particle tracking size and concentration measurements. While such background intensity can be avoided with light sheet illumination, current set-ups require specialty sample holders that are not compatible with high-throughput diagnostics. Here, a microfluidic chip with integrated light sheet illumination is reported, and accurate fluorescence single particle tracking size and concentration measurements of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours are demonstrated.

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Citations

Apr 11, 2015·Advanced Drug Delivery Reviews·Benjamin S SchusterJustin Hanes
Apr 4, 2015·Optics Express·Øystein Ivar HelleOlav Gaute Hellesø
Aug 6, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Stephan StremerschKoen Raemdonck
Apr 1, 2016·Lab on a Chip·Petra PaièRoberto Osellame
Jan 13, 2018·Microscopy Research and Technique·Zagato ElisaBraeckmans Kevin
Sep 14, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Petra PaièAndrea Bassi
Feb 15, 2019·Frontiers in Neuroanatomy·Ignacio Albert-SmetJorge Ripoll
Oct 30, 2020·American Journal of Reproductive Immunology : AJRI·Swasti TiwariSantosh K Verma
May 28, 2020·Medical Hypotheses·Ahmet Topuzoğlu, Can Ilgın
Jan 17, 2021·Journal of Biophotonics·Zengxin HuangXizeng Feng
Jan 21, 2021·Frontiers in Veterinary Science·Bahram TalebjediMarcia De Almeida Monteiro Melo Ferraz
Dec 29, 2020·American Journal of Physiology. Cell Physiology·Samantha Peel, Mark Jackman
Jun 15, 2021·Lab on a Chip·Erick J Vargas-OrdazVictor J Cadarso

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