On-Chip Multiple Particle Velocity and Size Measurement Using Single-Shot Two-Wavelength Differential Image Analysis

Micromachines
Shuya SawaKenji Yasuda

Abstract

Precise and quick measurement of samples' flow velocities is essential for cell sorting timing control and reconstruction of acquired image-analyzed data. We developed a simple technique for the single-shot measurement of flow velocities of particles simultaneously in a microfluidic pathway. The speed was calculated from the difference in the particles' elongation in an acquired image that appeared when two wavelengths of light with different irradiation times were applied. We ran microparticles through an imaging flow cytometer and irradiated two wavelengths of light with different irradiation times simultaneously to those particles. The mixture of the two wavelength transmitted lights was divided into two wavelengths, and the images of the same microparticles for each wavelength were acquired in a single shot. We estimated the velocity from the difference of its elongation divided by the difference of irradiation time by comparing these two images. The distribution of polystyrene beads' velocity was parabolic and highest at the center of the flow channel, consistent with the expected velocity distribution of the laminar flow. Applying the calculated velocity, we also restored the accurate shapes and cross-sectional areas of p...Continue Reading

References

Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·H P ChouS Quake
Jun 5, 2004·Journal of Nanobiotechnology·Kazunori TakahashiKenji Yasuda
Mar 31, 2005·Physiological Measurement·Dongeun HuhShuichi Takayama
Apr 13, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Karen CheungPhilippe Renaud
Jun 29, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Karen C CheungAttila Tárnok
Oct 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shannon L StottMehmet Toner
Jul 13, 2011·International Journal of Molecular Sciences·Masahito HayashiKenji Yasuda
Mar 1, 2014·Nature Protocols·Nezihi Murat KarabacakMehmet Toner
Jan 23, 2016·Molecular Oncology·Kiki C AndreeLeon W M M Terstappen
Jan 13, 2018·Advanced Drug Delivery Reviews·Si-Jie HaoSi-Yang Zheng
Apr 25, 2018·Analytical Chemistry·Ramesh UtharalaChristoph A Merten
Jun 16, 2018·Science·Sadao OtaHiroyuki Noji
Sep 1, 2018·Cell·Nao NittaKeisuke Goda
May 28, 2020·Scientific Reports·Muhsincan Sesen, Graeme Whyte
Oct 9, 2020·Biosensors & Bioelectronics·Srinivasu Valagerahally PuttaswamyJames McLaughlin

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
irradiate
chip
Flow

Software Mentioned

Python

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Jin ZuoDonald B Twieg
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Svetoslav Ivanov Nikolov, Jørgen Arendt Jensen
Journal of the American College of Cardiology
M MathisonK Asano
© 2021 Meta ULC. All rights reserved