On chip two-photon metabolic imaging for drug toxicity testing

Biomicrofluidics
Fang YuHanry Yu

Abstract

We have developed a microfluidic system suitable to be incorporated with a metabolic imaging method to monitor the drug response of cells cultured on a chip. The cells were perfusion-cultured to mimic the blood flow in vivo. Label-free optical measurements and imaging of nicotinamide adenine dinucleotide and flavin adenine dinucleotide fluorescence intensity and morphological changes were evaluated non-invasively. Drug responses calculated using redox ratio imaging were compared with the drug toxicity testing results obtained with a traditional well-plate system. We found that our method can accurately monitor the cell viability and drug response and that the IC50 value obtained from imaging analysis was sensitive and comparable with a commonly used cell viability assay: MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium) assay. Our method could serve as a fast, non-invasive, and reliable way for drug screening and toxicity testing as well as enabling real-time monitoring of in vitro cultured cells.

References

Apr 6, 1990·Science·W DenkW W Webb
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·N Ramanujam
Apr 20, 2002·Biophysical Journal·Shaohui HuangWatt W Webb
Jul 18, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Stefan WilkeningAugustinus Bader
Nov 5, 2003·Nature Biotechnology·Warren R ZipfelWatt W Webb
Dec 12, 2003·Journal of Neurophysiology·Michael J LeveneWatt W Webb
Apr 5, 2005·Toxicologic Pathology·Donna M DambachFrederic Moulin
Mar 7, 2006·Nature Reviews. Drug Discovery·Petra S Dittrich, Andreas Manz
Jul 28, 2006·Nature·Jamil El-AliKlavs F Jensen
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Melissa C SkalaNirmala Ramanujam
Jan 1, 2008·Journal of Biomedical Optics·William L RiceIrene Georgakoudi
May 23, 2009·Science·Matthew G Vander HeidenCraig B Thompson
Jul 2, 2009·Lab on a Chip·Yi-Chin TohHanry Yu
Feb 4, 2010·Lab on a Chip·Jong Hwan SungMichael L Shuler
May 13, 2010·Cancer Research·Julie Hanson OstranderNimmi Ramanujam
Oct 26, 2010·Biomaterials·Shufang ZhangHanry Yu
Feb 22, 2011·Biotechnology and Bioengineering·Jean Matthieu ProtEric Leclerc
Apr 8, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mothanna Al-QubaisiNoorjahan B Alitheen
May 4, 2011·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Nam T VuArion F Chatziioannou
Oct 29, 2011·Trends in Cell Biology·Dongeun HuhDonald E Ingber
Aug 2, 2012·Nature Reviews. Drug Discovery·Pavel NeužiAndreas Manz
Nov 3, 2012·Journal of Biomedical Optics·Alex J WalshMelissa C Skala
Nov 9, 2012·Science Translational Medicine·Dongeun HuhDonald E Ingber
Feb 28, 2013·Lab on a Chip·Birgit UngerböckTorsten Mayr
Aug 6, 2014·Nature Biotechnology·Sangeeta N Bhatia, Donald E Ingber
Dec 29, 2015·Biomaterials·Wen Hao TongHanry Yu
Jun 13, 2016·ACS Biomaterials Science & Engineering·Layla AlhasanLeslie Y Yeo

❮ Previous
Next ❯

Citations

Nov 27, 2019·Biomicrofluidics·Jiahui SunXianting Ding
Mar 2, 2019·Micromachines·Fang YuDeepak Choudhury
Nov 6, 2020·Developmental Biology·Nicola GrittiVikas Trivedi
Dec 29, 2020·American Journal of Physiology. Cell Physiology·Samantha Peel, Mark Jackman
Mar 17, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kirsten Eckstrum, Robert Sprando
Apr 1, 2021·Stem Cells·Laleh ShariatiAbbas Amini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.