PMID: 11340975May 9, 2001Paper

On-column amperometric detection of ofloxacin and pasiniazid in urine by capillary electrophoresis with an improved fractured joint and small detection cell

The Analyst
S S ZhangC L Yu

Abstract

An improved high-voltage electric field isolating joint and small detection cell have been carefully designed and fabricated. The joint possesses short steady time, high electric conductance efficiency and high performance. The cell is convenient to install and remove the capillaries with and without the joint, as well as to fix, adjust and insert the microelectrode into the detection capillary. Using the joint and the cell, an analytical method for determination of ofloxacin (Oflx) and pasiniazid (Ipa) in urine by capillary electrophoresis with on-column amperometric detection was developed. The calibration lines were linear in the range of 10-100 mg l-1 of Oflx and 1.0-50 mg l-1 of Ipa, respectively. The detection limits were 8.5 mg l-1 of Oflx and 0.80 mg l-1 of Ipa. Their recovery ranged from 101 to 104%. The accuracy and intra-day and inter-day reproducibility of Oflx and Ipa were determined with satisfactory results. This method was successfully used for determining Oflx and Ipa in human urine.

Citations

Nov 15, 2002·Electrophoresis·Lisa A Holland, Alyison M Leigh
Jan 14, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takako YamaguchiYoshikazu Fujita
Jul 18, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Zhanguang ChenGuoliang Liu
Jul 12, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·M Soledad GarcíaMustafa Salem Abuherba
May 1, 2009·Bioanalysis·Marilyn J Schneider
Dec 8, 2004·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xia WuYuxia Luan
Jul 16, 2019·Physical Chemistry Chemical Physics : PCCP·Ting LiuYumin Qian

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