On-column derivatization with o-phthaldialdehyde for fast determination of homocysteine in human urine

Analytical and Bioanalytical Chemistry
Rafał GłowackiHieronim Jakubowski

Abstract

A new assay for urinary homocysteine is described. The assay relies on an on-column derivatization with o-phthaldialdehyde, using a reversed-phase HPLC column and detection/quantification by fluorescence. The analysis time for reduced and total homocysteine, including sample work-up, was 5 and 13 min, respectively. Quantification limit was 25 nM.

References

Mar 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yoshio MukaiShinzo Tanabe
Dec 4, 2004·Clinical Chemistry·Grazyna Chwatko, Hieronim Jakubowski
Jun 23, 2006·Analytical and Bioanalytical Chemistry·K KuśmierekE Bald
Feb 8, 2007·Amino Acids·J Perła-KajánH Jakubowski
May 30, 2008·Clinical Chemistry·Per Magne Ueland
Jun 24, 2008·Analytical Biochemistry·Hieronim Jakubowski
Oct 31, 2008·Talanta·Olga NekrassovaRichard G Compton
Jun 27, 2009·Clinical Chemistry·Donald W JacobsenL M Taylor

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Citations

May 30, 2015·Chemical Reviews·Justyna M Płotka-WasylkaJacek Namieśnik

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