On-demand delivery of single DNA molecules using nanopipets

ACS Nano
Aleksandar P IvanovJoshua B Edel

Abstract

Understanding the behavioral properties of single molecules or larger scale populations interacting with single molecules is currently a hotly pursued topic in nanotechnology. This arises from the potential such techniques have in relation to applications such as targeted drug delivery, early stage detection of disease, and drug screening. Although label and label-free single molecule detection strategies have existed for a number of years, currently lacking are efficient methods for the controllable delivery of single molecules in aqueous environments. In this article we show both experimentally and from simulations that nanopipets in conjunction with asymmetric voltage pulses can be used for label-free detection and delivery of single molecules through the tip of a nanopipet with "on-demand" timing resolution. This was demonstrated by controllable delivery of 5 kbp and 10 kbp DNA molecules from solutions with concentrations as low as 3 pM.

References

Apr 2, 2002·Analytical Chemistry·Liming YingDavid Klenerman
Nov 22, 2002·Physical Review Letters·Z Siwy, A Fuliński
Mar 30, 2005·Nano Letters·Miloslav KarhanekChris D Webb
Sep 24, 2005·Nano Letters·Arnold J StormCees Dekker
Aug 28, 2007·Nano Letters·Guillaume A T ChansinJoshua B Edel
Jan 30, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Henry S White, Andreas Bund
Jul 26, 2008·Nature Nanotechnology·Cees Dekker
Jul 26, 2008·Nature Nanotechnology·Samir M IqbalRashid Bashir
Jul 26, 2008·Nature Nanotechnology·Marc Gershow, J A Golovchenko
Mar 3, 2009·Nature Methods·Pavel NovakYuri E Korchev
Dec 22, 2009·Nature Nanotechnology·Meni WanunuAmit Meller
Jun 3, 2010·Nano Letters·Lorenz J SteinbockUlrich F Keyser
Dec 8, 2010·Nano Letters·Aleksandar P IvanovJoshua B Edel
Dec 15, 2010·Nature Nanotechnology·Riju SinghalYury Gogotsi
Aug 2, 2011·Journal of the American Chemical Society·Wen-Jie LanHenry S White
Sep 20, 2011·Nature Nanotechnology·Bala Murali Venkatesan, Rashid Bashir
Nov 10, 2011·ACS Nano·Meni WanunuMarija Drndic
Mar 13, 2012·Nature Nanotechnology·Ruoshan WeiUlrich Rant
Aug 18, 2012·Nanoscale·R Adam SegerNader Pourmand
Sep 20, 2012·Chemical Society Reviews·Benjamin N MilesJoshua B Edel
Nov 14, 2012·Electrophoresis·Lorenz J SteinbockUlrich F Keyser
Apr 9, 2013·Lab on a Chip·Nicholas A W BellUlrich F Keyser
Apr 24, 2013·ACS Nano·Wenhong LiUlrich F Keyser
Sep 6, 2013·Analytical Chemistry·Babak BabakinejadYuri E Korchev
Nov 28, 2013·ACS Nano·Paolo ActisNader Pourmand
Dec 18, 2013·Analytical Chemistry·Xiuqing GongJoshua B Edel
Jan 1, 2014·ACS Nano·Paolo ActisYuri E Korchev
Jan 9, 2014·Analytical Chemistry·Thomas R GibbTim Albrecht
Nov 19, 2014·Analytical Chemistry·Daniel G HaywoodStephen C Jacobson

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Citations

Jan 9, 2016·Biophysical Journal·Sophie SchobesbergerJulia Gorelik
Jan 7, 2016·Nature Communications·Kevin J FreedmanJoshua B Edel
Jul 25, 2015·ACS Applied Materials & Interfaces·Colin R CrickJoshua B Edel
Mar 18, 2016·Nanoscale·Raquel L FraccariTim Albrecht
Apr 26, 2016·Analytical Chemistry·David PerryPatrick R Unwin
Oct 11, 2016·Faraday Discussions·Yixian WangNongjian Tao
Jun 8, 2018·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Jian ZhuangMugubo Vincent
Nov 27, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hitoshi Shiku
Jul 11, 2019·The Analyst·Zhe WangShuai Chang
Jul 17, 2019·Nature Nanotechnology·Morteza ArameshJános Vörös
Oct 2, 2019·Assay and Drug Development Technologies·Sezer Okay
Sep 12, 2018·Beilstein Journal of Nanotechnology·Evelyne Salançon, Bernard Tinland
Jan 17, 2020·Advanced Materials·Stuart G HigginsMolly M Stevens
May 10, 2017·Proceedings. Mathematical, Physical, and Engineering Sciences·Ashley PagePatrick R Unwin
Sep 21, 2017·Nature Communications·Ren RenYuri Korchev
Oct 31, 2018·Biosensors·Marta Maria Pereira da Silva Neves, Daniel Martín-Yerga
Apr 18, 2017·Proceedings. Mathematical, Physical, and Engineering Sciences·Yixian WangMichael V Mirkin
Feb 18, 2021·Analytical Chemistry·Bing LiDavid Klenerman
Feb 25, 2021·Chemical Society Reviews·Jasper P FriedJames R Yates
Dec 3, 2019·Chemical Science·Xiaoyi WangJoshua B Edel
Jun 18, 2021·Lab on a Chip·Mahmudur RahmanHolger Schmidt
Mar 24, 2018·Nano Letters·Paolo CadinuAleksandar P Ivanov
Oct 8, 2019·Analytical Chemistry·Linhao SunShinji Watanabe
Oct 27, 2018·ACS Applied Materials & Interfaces·Liang XueJoshua B Edel
Jan 21, 2017·Analytical Chemistry·Wenqing ShiLane A Baker
Apr 20, 2019·Analytical Chemistry·Markéta KubánkováMarina K Kuimova
Apr 20, 2018·ACS Nano·Mojun ChenJi Tae Kim

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