PMID: 15387175Sep 25, 2004Paper

On-line characterisation of apple polyphenols by liquid chromatography coupled with mass spectrometry and ultraviolet absorbance detection

Journal of Chromatography. a
Rosa M Alonso-SalcesFrancisca Vicente

Abstract

Apple polyphenols were characterised by means of hyphenated techniques such as HPLC coupled to UV photodiode array detection (LC-DAD) and to mass spectrometry (LC-MS). LC-MS using atmospheric pressure ionisation (APCI) in the positive ion mode provided the molecular weight, the number of hydroxyl groups, the number of sugars and an idea about the substitution pattern of the polyphenols. LC-DAD with postcolumn addition of UV shift reagents afforded precise structural information about the position of the free hydroxyl groups in the polyphenolic nucleus. Five isorhamnetin glycosides, two hydroxyphloretin glycosides and quercetin were reported in apple peel for the first time. Postcolumn addition of UV shift reagents in LC-DAD analysis confirmed the presence of isorhamnetin glycosides and not the isomeric glycosides of rhamnetin. Moreover, isorhamnetin-3-O-rhamnoglucoside was identified unambiguously by comparison with a standard. These results are relevant not only from a chemotaxonomic point of view, but also in the control of authenticity of fruit derived products in order to detect fraudulent admixtures.

References

Feb 1, 1997·Critical Reviews in Food Science and Nutrition·T Beveridge
Aug 22, 2001·Journal of Agricultural and Food Chemistry·R M Alonso-SalcesF Vicente
Jan 5, 2002·Journal of Chromatography. a·R M Alonso-SalcesF Vicente

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Citations

Jul 30, 2010·Journal of Agricultural and Food Chemistry·Mustafa Çam, Kjersti Aaby
Mar 14, 2012·Journal of Agricultural and Food Chemistry·Sylvie BureauCatherine M G C Renard
Sep 28, 2012·Journal of Agricultural and Food Chemistry·Christelle M AndreWilliam A Laing
May 4, 2013·Journal of Agricultural and Food Chemistry·M José Jara-PalaciosCelestino Santos-Buelga
Apr 27, 2005·The Analyst·Warwick B DunnHelen E Johnson
Aug 4, 2012·Journal of Experimental Botany·Rebecca A Henry-KirkAndrew C Allan
Jul 26, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Jian GuoRichard B van Breemen
Apr 25, 2009·International Journal of Food Sciences and Nutrition·Denis R LaurenMargot A Skinner
Jul 28, 2009·Journal of Chromatography. a·Kathithileni M Kalili, André de Villiers
Jul 2, 2005·Molecular Nutrition & Food Research·Kathrin KahleElke Richling
Sep 19, 2009·Molecular Nutrition & Food Research·Mathias JungGerhard Erkel
Dec 12, 2007·Journal of Separation Science·Constantine D Stalikas
Jun 1, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A SinghA Mercenier
Aug 14, 2012·Journal of the Science of Food and Agriculture·Seyda KaramanReşat Apak
Mar 30, 2005·Journal of Chromatography. a·Belén SuárezRoberto Rodríguez
Apr 23, 2015·Journal of Photochemistry and Photobiology. B, Biology·Luana Beatriz Dos Santos NascimentoEliana Schwartz Tavares
Dec 1, 2012·Food Chemistry·Matthias FrommDietmar R Kammerer
Dec 1, 2011·Food Chemistry·Zhongxiang Fang, Bhesh Bhandari
Dec 3, 2014·Journal of Pharmaceutical and Biomedical Analysis·Su SuMing Xue
Oct 30, 2016·British Journal of Pharmacology·Antonello Santini, Ettore Novellino
Feb 12, 2017·Journal of the Science of Food and Agriculture·Barbara KusznierewiczAgnieszka Bartoszek
Jul 12, 2018·Journal of Mass Spectrometry : JMS·Stefania SutStefano Dall'Acqua
Jan 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuan LiJin-Ao Duan
Jun 3, 2005·Phytochemical Analysis : PCA

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