On-line control of an immobilized hybridoma culture with multi-channel flow injection analysis

Journal of Biotechnology
J J van der PolC Wandrey

Abstract

An immobilized hybridoma cell line was cultivated at controlled glucose and glutamine concentrations. On-line analysis of the substrates was carried out with a multi-channel flow injection analysis system. The analysis system also determined on-line the lactate and ammonium concentration. The substrate concentrations were controlled using an adaptive-control strategy. This strategy consisted of the estimation of the real-time concentrations and volumetric substrate consumption rates by an Extended Kalman Filter, and a minimum variance controller, which used the estimated parameters to set the feed rates of the substrates. The closed-loop control was used to start-up two cultures with either glucose or glutamine as control-substrate for the medium feed rate. The controller kept the concentration of the control-substrate constant by enhancing the medium feed rate simultaneously to the increasing volumetric consumption rate of the substrate. When glutamine was used as control-substrate, the glucose concentration remained relatively constant, whereas the glutamine concentration decreased during the start-up at a constant glucose concentration. This indicates that glutamine is consumed faster than glucose and will be a better contro...Continue Reading

References

Jan 1, 1994·Cytotechnology·I LüdemannH Märkl
Nov 15, 1994·Journal of Biotechnology·J J van der PolJ Tramper
Aug 5, 1988·Biotechnology and Bioengineering·M W GlackenA J Sinskey
May 1, 1990·Biotechnology and Bioengineering·A McQueen, J E Bailey
Feb 20, 1992·Biotechnology and Bioengineering·S S OzturkB O Palsson
Nov 20, 1993·Biotechnology and Bioengineering·E C JoH M Moon
Jan 5, 1994·Biotechnology and Bioengineering·K A HawboldtL A Behie
Mar 5, 1994·Biotechnology and Bioengineering·M NewlandL K Nielsen
Sep 5, 1994·Biotechnology and Bioengineering·R P SinghA N Emery
Sep 5, 1994·Biotechnology and Bioengineering·D K RobinsonM Silberklang
Sep 20, 1994·Biotechnology and Bioengineering·J Ljunggren, L Häggström

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.