PMID: 12766572May 27, 2003Paper

On-line derivatization utilizing solid-phase microextraction (SPME) for determination of busulphan in plasma using gas chromatography-mass spectrometry (GC-MS)

Therapeutic Drug Monitoring
Mohamed Abdel-RehimMoustapha Hassan

Abstract

Busulphan (Bu) is an alkylating agent used in preparative regimen before stem cell transplantation (SCT). Bu has a narrow therapeutic window, and underdosing or overdosing may have a fatal outcome for the patient. Therapeutic drug monitoring (TDM) combined with dose adjustment is currently used to optimize and individualize therapy with Bu. However, this approach is limited to centers with laboratory facilities. An automated and easy method for measurement of Bu plasma concentrations may facilitate TDM for Bu and thus improve the clinical outcome. A solid-phase microextraction (SPME) on line with gas chromatography (GC) and mass-spectrometric detection to quantify Bu in human plasma samples was developed using in-vial derivatization. Bu was mixed with reagent in a 2-mL vial and shaken for 15 minutes at 80 degrees C; subsequently, the SPME fiber was immersed into the vial for 15 minutes. The fiber was washed in water for 10 seconds before injection. Several parameters influencing the extraction and recovery were studied, such as absorption and desorption times, the effects of the temperature on the reaction, and the shaking time on the derivatization yield. Carbowax-divinylbenzene, polyacrylate, and polydimethylsiloxane fibers w...Continue Reading

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Citations

Sep 4, 2009·Analytical and Bioanalytical Chemistry·Hiroyuki Kataoka
Apr 2, 2014·Journal of Analytical Methods in Chemistry·Mohammad Mahdi MoeinMohamed Abdel-Rehim
May 1, 2007·Analytical and Bioanalytical Chemistry·Sarah M R Wille, Willy E E Lambert
Nov 5, 2003·Journal of Mass Spectrometry : JMS
May 6, 2011·Therapeutic Drug Monitoring·JoEtta M JuenkeKamisha L Johnson-Davis
Sep 9, 2011·Bioanalysis·Nadia Y Ashri, Mohamed Abdel-Rehim
Sep 24, 2015·Bioanalysis·Mohammad Mahdi MoeinMohamed Abdel-Rehim
May 4, 2007·Analytical and Bioanalytical Chemistry·Fritz Pragst
Jun 1, 2017·The Analyst·Nicolas GuichardSandrine Fleury-Souverain
May 28, 2014·Biomedical Chromatography : BMC·Jorge PereiraMohamed Abdel-Rehim
Mar 5, 2021·Biomedical Chromatography : BMC·Seyed Mosayeb DaryanavardMohamed Abdel-Rehim
May 13, 2021·Annual Review of Analytical Chemistry·Suji LeeAbraham K Badu-Tawiah

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