On-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development

Bioprocess and Biosystems Engineering
Bence KozmaSzilveszter Gergely

Abstract

NIR spectroscopy is a non-destructive tool for in-situ, on-line bioprocess monitoring. One of its most frequent applications is the determination of metabolites during cultivation, especially glucose. Previous studies have usually investigated the applicability of Near Infrared (NIR) spectroscopy at one bioreactor scale but the effect of scale up was not explored. In this study, the complete scale up from shake flask (1 L) through 20 L, 100 L and 1000 L up to 5000 L bioreactor volume level was monitored with on-line NIR spectroscopy. The differences between runs and scales were examined using principal component analysis. The bioreactor runs were relatively similar regardless of scales but the shake flasks differed strongly from bioreactor runs. The glucose concentration throughout five 5000 L scale bioreactor runs were predicted by partial least squares regression models that were based on pre-processed spectra of bioreactor runs and combinations of them. The model that produced the lowest error of prediction (4.18 mM on a 29 mM concentration range) for all five runs in the prediction set was based on the combination of 20 L and 100 L data. This result demonstrated the capabilities and the limitations of an NIR system for gluc...Continue Reading

References

Aug 12, 2003·Biotechnology and Bioengineering·S Alison ArnoldBrian McNeil
Jun 23, 2009·Advances in Biochemical Engineering/biotechnology·João G HenriquesJosé C Menezes
May 6, 2010·Analytica Chimica Acta·Zou XiaoboMao Hanpin
Aug 10, 2010·Acta Pharmacologica Sinica·Jing Li, Zhenping Zhu
Sep 19, 2015·Critical Reviews in Biotechnology·Jennifer DumontRashmi Kshirsagar

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Citations

Aug 29, 2021·Current Opinion in Biotechnology·Coral Fung Shek, Michael Betenbaugh

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Methods Mentioned

BETA
light scattering
PCA

Software Mentioned

Simatic SiPAT
PLS
Matlab
iPLS
_ Toolbox
PCA

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