PMID: 2094419Jan 1, 1990Paper

On-line mass spectrometric investigation of the peroxidase-catalysed oxidation of uric acid

Journal of Pharmaceutical and Biomedical Analysis
K J VolkA Brajter-Toth

Abstract

The enzymatic and electrochemical oxidation pathways of uric acid were determined on-line with thermospray-tandem mass spectrometry. Products and intermediates formed as a result of electrooxidation were monitored as the electrode potential was varied. Electrochemical results served as a model for the enzymatic studies. In fact, electrochemical studies were essential for elucidating the structures of intermediates because of the high conversion efficiencies in electrooxidation. Products and intermediates formed as a result of enzymatic oxidation of uric acid were monitored as the reaction time was varied. When the enzymatic oxidation of uric acid with peroxidase and H2O2 was studied, the same intermediates and products were observed as in the electrochemical oxidation. The tandem mass spectrometric results provide convincing evidence that the primary intermediate produced during both the enzymatic and electrochemical oxidation of uric acid has a quinonoid diimine structure. The primary intermediate can follow three distinct reaction pathways to produce the identified final products. The final enzymatic and electrochemical oxidation products observed in these studies were urea, CO2, alloxan, alloxan monohydrate, allantoin, 5-hyd...Continue Reading

References

Dec 1, 1986·Analytical Chemistry·P Rudewicz, K M Straub
Feb 26, 1982·Biochemical and Biophysical Research Communications·J R Rice, P T Kissinger

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Citations

Aug 1, 1992·Journal of Biotechnology·G LocherA Fiechter
Jan 27, 2011·The Journal of Biological Chemistry·Flavia C MeottiAnthony J Kettle
Jul 19, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Roman Kand'ár, Pavla Záková
Oct 1, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Roman Kand'árVladimíra Muzáková
Apr 18, 2018·Journal of Separation Science·Feipeng DuanHongbin Xiao
Nov 6, 2018·The Journal of Biological Chemistry·Rufus TurnerAnthony J Kettle
Oct 13, 2009·Toxicology Mechanisms and Methods·Achuthan C RaghavamenonRao M Uppu
Jun 15, 2014·The Journal of Biological Chemistry·Antonia SeidelGuy N L Jameson
Jun 2, 2015·Journal of the American Chemical Society·Timothy A BrownRichard N Zare

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