On-line metal chelate affinity chromatography clean-up for the high-performance liquid chromatographic determination of tetracycline antibiotics in animal tissues

Journal of Chromatography. B, Biomedical Applications
G W StubbingsG Shearer

Abstract

An on-line high-performance liquid chromatographic (HPLC) method for the determination of tetracycline, oxytetracycline, chlortetracycline and demeclocycline using metal chelate affinity chromatography-reversed-phase HPLC has been developed. The drugs were extracted with succinate buffer and the extract diluted with EDTA-pentanesulphonate buffer. Diluted extract was then absorbed onto a C8 or XAD-2 solid-phase extraction (SPE) cartridge and eluted with methanol. The eluate was then injected onto a TSKgel chelate column which had been preloaded with copper(II). The tetracyclines were eluted from this column onto the analytical column (Polymer Labs. PLRP-S) with an EDTA-containing buffer. Elution of the analytical column was via a methanol-acetonitrile gradient and detection was by UV at 350 nm. Average recoveries at the 10, 20, 50 and 300 micrograms kg-1 levels were 50-80%. The limit of detection (LOD) was 10 micrograms kg-1 for oxytetracycline and tetracycline and 20 micrograms kg-1 for chlortetracycline and demeclocycline. The method was validated for sheep liver and cattle kidney.

Citations

Jul 18, 2008·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Kazuhiro FujitaMakoto Taniguchi
Oct 22, 2016·Critical Reviews in Analytical Chemistry·Rúbia Adrieli SversutHérida Regina Nunes Salgado
Oct 22, 2013·Journal of Environmental Management·José Rivera-UtrillaRaúl Ocampo-Pérez
Feb 12, 2000·Rapid Communications in Mass Spectrometry : RCM·N Van EeckhoutC Van Peteghem
May 3, 2005·Journal of Pharmaceutical and Biomedical Analysis·M C MahederoB Roldán
Jul 13, 2000·Journal of Chromatography. a·L Bovanová, E Brandsteterová
Jul 15, 2000·Journal of Chromatography. a·H OkaH Matsumoto
Jun 25, 2005·Journal of Chromatography. a·Collin R AndersonWen-Hsin Wu
Nov 26, 2004·Journal of Agricultural and Food Chemistry·Guoying Chen, Linshu Liu

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