On-line three-parameter data uptake, analysis, and display device for flow cytometry and other applications

Cytometry
V Kachel, H Schneider

Abstract

Multiparameter flow cytometric measurements are of growing interest in the study of complex features of biological cells. With state of the art instrumentation, three-parameter (3-P) data handling is relatively complicated and time consuming and the display methods are not satisfactory. As an alternative, an interactive 3-P analyzing module, Cytomic 123 is described, which displays 3-P fields during and immediately after data uptake in the form of a cubic array of 32,768 channels. The fields can be randomly rotated by hardware and software. The event frequencies in the field are primarily visualized by brightness modulation of the display dots. Additionally, the display of the field may be confined to user selected ranges of event frequencies, which may also be superposed to mixed frequency displays. A set of preprogrammed functions is available for the following tasks: (a) uptake of 3-P histograms combined with on-line control of the transducer pulses, (b) automatic uptake of a series of 2-P time correlated histograms in the cube, (c) generation and numerical evaluation of sections and projections of cube histograms, (d) interactive generation and evaluation of spatial subfields for integration, or as sorting matrix by success...Continue Reading

Citations

Jan 1, 1995·Journal of Reproductive Immunology·N M BhatN N Teng
Jul 1, 1988·Computer Methods and Programs in Biomedicine·P M SlootC G Figdor
Feb 1, 1994·Computer Methods and Programs in Biomedicine·W Beisker
Jan 1, 1990·Cytometry·V KachelP Hummel
Jan 1, 1989·Cytometry·P M Sloot, C G Figdor
Mar 1, 1987·Cytometry·M G Ormerod, A W Payne

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