On-resin assembly of a linkerless lanthanide(III)-based luminescence label and its application to the total synthesis of site-specifically labeled mechanosensitive channels

Bioconjugate Chemistry
Christian F W BeckerGerd G Kochendoerfer

Abstract

A synthesis strategy for the on-resin assembly of luminescent lanthanide chelates from commercially available compounds was developed. Advantages of the approach include the absence of spacers between the metal ion and the attachment site, and the compatibility with typical chemical protein synthesis protection schemes. Methoxycoumarin-labeled lysine and tris(tert-butyl)-DOTA were consecutively coupled with high efficiency to a free amino group in otherwise fully protected peptide segments using standard peptide synthesis methods. Addition of stoichiometric amounts of Tb(3+) to the modified, cleaved, and purified peptides yielded the desired lanthanide chelate. Incorporation of this label into a chemically synthesized, full-length mechanosensitive channel of large conductance (MscL) of E. coli and subsequent reconstitution into vesicles resulted in a functional mechanosensitive channel of comparable conductance to the wild-type channel. However, this channel required increased suction to gate. Excitation of the antenna molecule methoxycoumarin at 336 nm resulted in an emission spectrum typical for Tb(3+) and a luminescence lifetime of 0.67 ms. The location of the probe close to the backbone of this protein may provide precise i...Continue Reading

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Citations

Sep 17, 2005·Journal of Fluorescence·I Hemmilä, V Laitala
Mar 3, 2007·Bioconjugate Chemistry·Byunghee Yoo, Mark D Pagel
Sep 29, 2005·Biomolecular Engineering·Thomas Durek, Christian F W Becker
May 17, 2014·Bioconjugate Chemistry·Kathrin BrücknerMichael W Linscheid
Mar 25, 2011·Angewandte Chemie·Sunanda LahiriChristian F W Becker
Jul 5, 2007·Luminescence : the Journal of Biological and Chemical Luminescence·Kazuki AitaHideo Saji
Aug 23, 2007·Contrast Media & Molecular Imaging·Byunghee YooMark D Pagel
Jun 20, 2008·Molecular BioSystems·Diana Olschewski, Christian F W Becker
Jun 27, 2019·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Qun WangCongjie Gao
Oct 28, 2017·Scientific Reports·George ShapovalovNatalia Prevarskaya
Aug 24, 2012·Chemical Communications : Chem Comm·Manickasundaram SamiappanGonen Ashkenasy

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