PMID: 9438381Jan 23, 1998Paper

On the adsorption of hyaluronan and ICAM-1 to modified hydrophobic resins

The International Journal of Biochemistry & Cell Biology
P McCourt, S Gustafson

Abstract

Hyaluronan is a negatively charged glycosaminoglycan that occurs in connective tissue and has a wide range of mechanical and cell biological functions. The purpose of this study was to utilize affinity chromatography resins for purification of detergent (Triton X-100) solubilized hyaluronan binding proteins from liver, the major organ of hyaluronan clearance from the blood. However, during these studies we made the unexpected finding that hyaluronan binds to Sepharose substituted with a hexamethylene chain, a commonly used spacer arm in affinity chromatography resins, capped with either a terminal primary amine or a terminal acetoamido group. Hyaluronan did not bind the hydrophobic resins hexyl- or octyl-Sepharose under the same conditions. It was also found that rat liver intercellular adhesion molecule-1 binds to resins containing the hexamethylene spacer arm, an interaction which could be inhibited with free hyaluronan oligosaccharides. Finally, we have determined that resins with ethylene spacer arms show no affinity for hyaluronan and can therefore be used to immobilize hyaluronan for chromatography of hyaluronan binding proteins. By using this resin we have purified two proteins of approximately 200 and 400 kDa from rat l...Continue Reading

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Citations

Dec 22, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·P A McCourt
Feb 7, 2002·The Biochemical Journal·Oliver PolitzSergij Goerdt
Aug 16, 2002·Molecular Biology of the Cell·Bin ZhouPaul H Weigel
Apr 11, 2001·Molecular Biology of the Cell·P BonoR O Hynes
Sep 3, 1998·The Journal of Clinical Investigation·J MollP Herrlich
Oct 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P A McCourtS Johansson
Feb 13, 2001·The Journal of Investigative Dermatology·T AhrensJ C Simon
Jun 14, 2002·Biochemical and Biophysical Research Communications·Janet A WeigelPaul H Weigel
Mar 20, 2014·Journal of Cellular Biochemistry·Daniel HagenfeldPeter Prehm
Nov 24, 1999·The Journal of Biological Chemistry·B ZhouP H Weigel
Aug 23, 2000·The Journal of Biological Chemistry·B ZhouP H Weigel
Nov 17, 2006·The Journal of Physical Chemistry. B·Valentina BusiniCarlo Cavallotti

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