On the antigenic determinants of the lipopolysaccharides of Vibrio cholerae O:1, serotypes Ogawa and Inaba.

The Journal of Biological Chemistry
J WangC P Glaudemans

Abstract

Monoclonal, murine IgG1s S-20-4, A-20-6, and IgA 2D6, directed against Vibrio cholerae O:1 Ogawa-lipopolysaccharide exhibited the same fine specificities and similar affinities for the synthetic methyl alpha-glycosides of the (oligo)saccharide fragments mimicking the Ogawa O-polysaccharide (O-PS). They did not react with the corresponding synthetic fragments of Inaba O-PS. IgG1s S-20-4 and A-20-6 have absolute affinity constants for synthetic Ogawa mono- to hexasaccharides of from approximately 10(5) to approximately 10(6) M-1. For IgG1s S-20-4, A-20-6, and IgA 2D6, the nonreducing terminal residue of Ogawa O-PS is the dominant determinant, accounting for approximately 90% of the maximal binding energy shown by these antibodies. Binding studies of derivatives of the Ogawa monosaccharide and IgGs S-20-4 and A-20-6 revealed that the C-2 O-methyl group fits into a somewhat flexible antibody cavity and that hydrogen bonds involving the oxygen and, respectively, the OH at the 2- and 3-position of the sugar moiety as well as the 2'-position in the amide side chain are required. Monoclonal IgA ZAC-3 and IgG3 I-24-2 are specific for V. cholerae O:1 serotypes Ogawa/Inaba-LPS.1 The former did not show binding with members of either serie...Continue Reading

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Citations

May 7, 2004·Molecular Immunology·Thierry Cl LaurentXavier De Bolle
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Joanna Kubler-KielbRachel Schneerson
Aug 19, 2011·The Journal of Infectious Diseases·Mohammad AliJohn Clemens
Dec 12, 2012·Journal of Bacteriology·Jialiang XuBiao Kan
Feb 12, 2014·PLoS Neglected Tropical Diseases·Mohammad Murshid AlamEdward T Ryan
Jul 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·S VilleneuveP M Alzari
Nov 4, 2015·The Journal of Organic Chemistry·Sameh E Soliman, Pavol Kováč
Jun 1, 2006·Clinical and Experimental Immunology·E PaulovicováS Bystrický
Nov 2, 2006·American Journal of Reproductive Immunology : AJRI·William F Wade
Jun 3, 2015·Glycobiology·Omid Haji-GhassemiStephen V Evans
Oct 3, 2006·FEMS Immunology and Medical Microbiology·Terri K WadeWilliam F Wade
Dec 13, 2007·Journal of Computational Chemistry·Anthony D Hill, Peter J Reilly
Jun 17, 2016·PLoS Neglected Tropical Diseases·Wilfredo R MatiasJason B Harris
Apr 13, 2021·ACS Infectious Diseases·Denise ChacAna A Weil
Aug 22, 2001·Carbohydrate Research·E PoirotP Kovác
Oct 16, 2003·Biochimica Et Biophysica Acta·S N Chatterjee, Keya Chaudhuri
Sep 28, 2005·Biochimica Et Biophysica Acta·S N Chatterjee, Keya Chaudhuri

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