On the complexation of proteins and polyelectrolytes

The Journal of Physical Chemistry. B
Fernando Luís B da SilvaTorbjörn Akesson

Abstract

Both natural and synthetic polyelectrolytes form strong complexes with a variety of proteins. One peculiar phenomenon is that association can take place even when the protein and the polyelectrolyte carry the same charge. This has been interpreted as if the ion-dipole interaction can overcome the repulsive ion-ion interaction. On the basis of Monte Carlo simulations and perturbation theory, we propose a different explanation for the association, namely, charge regulation. We have investigated three different protein-polymer complexes and found that the induced ionization of amino acid residues due to the polyelectrolyte leads to a surprisingly strong attractive interaction between the protein and the polymer. The extra attraction from this charge-induced charge interaction can be several kT and is for the three cases studied here, lysozyme, alpha-lactalbumin, and beta-lactoglobulin, of the same magnitude or stronger than the ion-dipole interaction. The magnitude of the induced charge is governed by a response function, the protein charge capacitance Z2-Z2. This fluctuation term can easily be calculated in a simulation or measured in a titration experiment.

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Citations

Jul 25, 2013·Quarterly Reviews of Biophysics·Mikael Lund, Bo Jönsson
Jan 30, 2010·International Journal of Molecular Sciences·Vincent Ball, Clarisse Maechling
Feb 10, 2011·The Journal of Chemical Physics·N Boon, R van Roij
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Mar 5, 2016·Annual Review of Food Science and Technology·Ty WagonerE Allen Foegeding
Mar 30, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Fu-Gen WuZhiwu Yu
Aug 19, 2015·The Journal of Chemical Physics·Cemil YigitJoachim Dzubiella
Jun 10, 2016·The Journal of Chemical Physics·Nataša Adžić, Rudolf Podgornik
Jul 6, 2016·Advances in Colloid and Interface Science·Yisheng XuYanchuan Guo
Apr 6, 2017·Journal of Chemical Theory and Computation·Fernando Luís Barroso da Silva, Donal MacKernan
Jan 18, 2020·Annual Review of Food Science and Technology·Fernando Luís Barroso da SilvaErik E Santiso
Sep 19, 2017·Biophysics Reviews·Fernando Luís Barroso daSilva, Luis Gustavo Dias
Mar 15, 2015·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Nataša Adžić, Rudolf Podgornik
May 9, 2019·Interface Focus·S PasqualiF L Barroso da Silva
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Oct 6, 2017·Biophysical Journal·Anže Lošdorfer Božič, Rudolf Podgornik
Apr 16, 2009·The Journal of Physical Chemistry. B·Sidney Jurado de CarvalhoFernando Luís Barroso da Silva
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Sep 2, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Deepti SrivastavaFernando Luís Barroso da Silva
Mar 30, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Qun-Li LeiRan Ni
Mar 4, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Fernando Luís Barroso da SilvaClas Persson
May 7, 2019·Journal of Chemical Theory and Computation·Fernando Luís Barroso da SilvaPhilippe Derreumaux
Apr 27, 2010·Journal of Colloid and Interface Science·Christian JohanssonPer Hansson
Nov 26, 2021·Physical Chemistry Chemical Physics : PCCP·Daniel L Z CaetanoSidney J de Carvalho

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