On the degradability and exocytosis of ceroid/lipofuscin in cultured rat cardiac myocytes

Mechanisms of Ageing and Development
Alexei Terman, U T Brunk

Abstract

The accumulation of lipofuscin (LF)--a polymeric, electron-dense, autofluorescent substance--within postmitotic cells is a characteristic manifestation of aging. It is generally believed that LF is undegradable and formed due to peroxidative alterations of various macromolecules under intralysosomal autophagic degradation. We report here that a short-term exposure of cultured neonatal rat cardiac myocytes to the thiol protease-inhibitor leupeptin, causes an accumulation of numerous electron-dense autophagic lysosomes within the cells. Although very similar to LF by ultrastructure, these inclusions do not display LF-specific, yellow-orange autofluorescence when excited with blue light. Moreover, they rapidly disappear from the cells upon re-establishment of normal culture conditions. In contrast, prolonged leupeptin treatment results in an accumulation of dense lysosomes that also show LF-typical autofluorescence. This autofluorescent material remains in the cells after the end of leupeptin action. The results suggest that: (i) a certain amount of time is needed for autophagocytosed material to become peroxidized, autofluorescent and undegradable, i.e. to acquire properties typical of LF; (ii) protease-inhibition by itself does ...Continue Reading

References

Feb 15, 1992·Experientia·P O Seglen, P Bohley
Mar 1, 1991·Mechanisms of Ageing and Development·G O IvyK Kitani
Jan 1, 1989·Free Radical Biology & Medicine·R S SohalU T Brunk
Nov 1, 1987·Chemical & Pharmaceutical Bulletin·K KikugawaK Sakurai
Apr 1, 1980·Journal of Biochemical and Biophysical Methods·D GalarisJ Rydström
Jun 30, 1995·Mechanisms of Ageing and Development·D YinU T Brunk
Apr 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·U WihlmarkS E Nilsson
Mar 1, 1991·Archives of Gerontology and Geriatrics·E A Porta

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Citations

Apr 5, 2002·Phytotherapy Research : PTR·Alexei Terman, Ulf T Brunk
Mar 12, 2013·Behavioural Brain Research·Jorge RodriguesJosé Paulo Andrade
Dec 15, 2005·Heart, Lung & Circulation·Alexei Terman, Ulf T Brunk
Feb 7, 2004·Archives of Gerontology and Geriatrics·Eduardo A PortaSebastian H Benavides
Feb 1, 2003·Experimental Gerontology·Valérie RenaultGillian Butler-Browne
Jun 28, 2002·Free Radical Biology & Medicine·Pamela A SzwedaLuke I Szweda
Sep 5, 2002·Free Radical Biology & Medicine·Ulf T Brunk, Alexei Terman
Oct 3, 2003·Ageing Research Reviews·Pamela A SzwedaLuke I Szweda
Feb 21, 2006·Antioxidants & Redox Signaling·Alexei Terman, Ulf T Brunk
Jan 8, 2009·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Chin-Yuan HsuYu-Pei Chan
Apr 9, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A Terman, U T Brunk
Apr 11, 2009·Microbial Cell Factories·Jacques M MathieuPedro Jj Alvarez
Aug 2, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hatice TohmaPeter G Arthur
Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ute FelborBjorn R Olsen
Dec 3, 2014·BMC Ophthalmology·Elizabeth M McElneaDeborah M Wallace
May 8, 2009·Cellular and Molecular Life Sciences : CMLS·Eun Seong HwangHyun Tae Kang
Jun 19, 2012·Heart Failure Reviews·José Marín-GarcíaGordon W Moe
Feb 14, 2016·Heart Failure Reviews·Gordon W Moe, José Marín-García
Jun 1, 2014·Experimental Gerontology·Kerstin NowotnyAnnika Höhn
Nov 9, 2014·Brain Structure & Function·Emmanuel P GilissenJean-Pierre Brion
Jul 13, 2004·Annals of the New York Academy of Sciences·Alexei TermanUlf T Brunk
Aug 25, 2004·The International Journal of Biochemistry & Cell Biology·Alexei Terman, Ulf T Brunk
May 3, 2001·Redox Report : Communications in Free Radical Research·A Terman
Jun 29, 2006·Annals of the New York Academy of Sciences·Alexei Terman
Oct 23, 2010·Experimental Gerontology·Yu-Shan Hsieh, Chin-Yuan Hsu
Jan 17, 2012·Neurobiology of Aging·Sylvie Julien, Ulrich Schraermeyer
Dec 21, 2004·Biochimica Et Biophysica Acta·C S M WongD R Richardson
Oct 14, 2014·Experimental Gerontology·Kerstin NowotnyAnnika Höhn
Jun 2, 2006·Chemico-biological Interactions·Alexei TermanUlf T Brunk
Feb 20, 2007·Archives of Biochemistry and Biophysics·Tino KurzUlf T Brunk
Oct 9, 2015·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·María Laura MessiOsvaldo Delbono
Jul 1, 2017·Stem Cells and Development·Monique M A VerstegenJeroen de Jonge
Aug 12, 2020·Multiple Sclerosis : Clinical and Laboratory Research·Megan L MorganAndrew V Caprariello
Jan 30, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Martin Dominik LeclairePeter Heiduschka
Jan 19, 2021·Journal of Diabetes Research·Nicholas EschEmilyn U Alejandro
Aug 14, 2003·Experimental Gerontology·Alexei TermanUlf T Brunk

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