On the Effects of Thermal History on the Development and Relaxation of Thermo-Mechanical Stress in Cryopreservation

Cryogenics
David P EisenbergYoed Rabin

Abstract

This study investigates the effects of the thermal protocol on the development and relaxation of thermo-mechanical stress in cryopreservation by means of glass formation, also known as vitrification. The cryopreserved medium is modeled as a homogeneous viscoelastic domain, constrained within either a stiff cylindrical container or a highly compliant bag. Annealing effects during the cooling phase of the cryopreservation protocol are analyzed. Results demonstrate that an intermediate temperature-hold period can significantly reduce the maximum tensile stress, thereby decreasing the potential for structural damage. It is also demonstrated that annealing at temperatures close to glass transition significantly weakens the dependency of thermo-mechanical stress on the cooling rate. Furthermore, a slower initial rewarming rate after cryogenic storage may drastically reduce the maximum tensile stress in the material, which supports previous experimental observations on the likelihood of fracture at this stage. This study discusses the dependency of the various stress components on the storage temperature. Finally, it is demonstrated that the stiffness of the container wall can affect the location of maximum stress, with implications o...Continue Reading

References

Dec 1, 1984·Cryobiology·I A JacobsenM P Diaper
Apr 1, 1996·Cryobiology·Y Rabin, P S Steif
Mar 1, 1997·Cryobiology·D E PeggS Boylan
Mar 4, 2000·Nature Biotechnology·Y C SongM J Taylor
Jun 21, 2006·Cryobiology·Yoed RabinMatthew C Palastro
Sep 26, 2006·Medical Engineering & Physics·Paul S SteifYoed Rabin
Sep 11, 2007·Annals of Biomedical Engineering·Jorge L Jimenez RiosYoed Rabin
Jan 11, 2008·Cell Preservation Technology·Paul S SteifYoed Rabin
Apr 17, 2008·Journal of Biomechanical Engineering·Paul S SteifYoed Rabin
Oct 30, 2008·Experimental Mechanics·Jorge L Jimenez Rios, Yoed Rabin

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Citations

Nov 26, 2015·Journal of Biomechanical Engineering·David P EisenbergYoed Rabin
Apr 4, 2015·Journal of Biomechanical Engineering·David P Eisenberg, Yoed Rabin
Jun 26, 2021·Journal of Applied Mechanics·Prem K Solanki, Yoed Rabin

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