On the Enigma of Glutathione-Dependent Styrene Degradation in Gordonia rubripertincta CWB2

Applied and Environmental Microbiology
Thomas HeineDirk Tischler

Abstract

Among bacteria, only a single styrene-specific degradation pathway has been reported so far. It comprises the activity of styrene monooxygenase, styrene oxide isomerase, and phenylacetaldehyde dehydrogenase, yielding phenylacetic acid as the central metabolite. The alternative route comprises ring-hydroxylating enzymes and yields vinyl catechol as central metabolite, which undergoes meta-cleavage. This was reported to be unspecific and also allows the degradation of benzene derivatives. However, some bacteria had been described to degrade styrene but do not employ one of those routes or only parts of them. Here, we describe a novel "hybrid" degradation pathway for styrene located on a plasmid of foreign origin. As putatively also unspecific, it allows metabolizing chemically analogous compounds (e.g., halogenated and/or alkylated styrene derivatives). Gordonia rubripertincta CWB2 was isolated with styrene as the sole source of carbon and energy. It employs an assembled route of the styrene side-chain degradation and isoprene degradation pathways that also funnels into phenylacetic acid as the central metabolite. Metabolites, enzyme activity, genome, transcriptome, and proteome data reinforce this observation and allow us to und...Continue Reading

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Citations

Mar 8, 2019·Biological Chemistry·Thomas HeineDirk Tischler
Mar 4, 2020·Microorganisms·Robin A DawsonJ Colin Murrell
Apr 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dirk TischlerThomas Heine
May 2, 2019·Frontiers in Microbiology·Dirk TischlerMarco W Fraaije
Dec 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Andrew T CrombieJ Colin Murrell
Aug 19, 2020·Applied Biochemistry and Biotechnology·Can CuiZhong-Liu Wu
Oct 28, 2020·Applied Biochemistry and Biotechnology·Juliane ZimmerlingDirk Tischler
Jul 31, 2021·Journal of Hazardous Materials·Pankaj BhattShaohua Chen
Jun 5, 2020·Microbiological Research·Ringo SchwabeDirk Tischler

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