On the functional interaction between nicotinic acetylcholine receptor and Na+,K+-ATPase.

Pflügers Archiv : European journal of physiology
Igor I KrivoiFrederic Mandel

Abstract

Previous studies have shown that nanomolar acetylcholine (ACh) produces a 2 to 4-mV hyperpolarization of skeletal muscle fibers putatively due to Na(+),K(+)-ATPase activation. The present study elucidates the involvement of the nicotinic ACh receptor (nAChR) and of Na(+),K(+)-ATPase isoform(s) in ACh-induced hyperpolarization of rat diaphragm muscle fibers. A variety of ligands of specific binding sites of nAChR and Na(+),K(+)-ATPase were used. Dose-response curves for ouabain, a specific Na(+),K(+)-ATPase inhibitor, were obtained to ascertain which Na(+),K(+)-ATPase isoform(s) is involved. The ACh dose-response relationship for the hyperpolarization was also determined. The functional relationship between these two proteins was also studied in a less complex system, a membrane preparation from Torpedo electric organ. The possibility of a direct ACh effect on Na(+),K(+)-ATPase was studied in purified lamb kidney Na(+),K(+)-ATPase and in rat red blood cells, systems where no nAChR is present. The results indicate that binding of nAChR agonists to their specific sites results in modulation of ouabain-sensitive (most probably alpha2) isoform of Na(+),K(+)-ATPase, leading to muscle membrane hyperpolarization. In the Torpedo prepara...Continue Reading

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Citations

Nov 30, 2012·The Journal of Biological Chemistry·Tatiana L RadzyukevichJudith A Heiny
Jan 10, 2012·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·V V KravtsovaI I Krivoi
May 20, 2008·American Journal of Physiology. Heart and Circulatory Physiology·John N LorenzJerry B Lingrel
Jan 13, 2016·The Journal of General Physiology·Violetta V KravtsovaIgor I Krivoi
Jul 22, 2009·Neuroscience Research·Haiping DengWolfgang Schwarz
Feb 6, 2015·BioMed Research International·Violetta V KravtsovaIgor I Krivoi
Jan 13, 2015·Bulletin of Experimental Biology and Medicine·V V KravtsovaI I Krivoi
Jun 3, 2016·Frontiers in Physiology·Vladimir V Matchkov, Igor I Krivoi
Feb 27, 2019·British Journal of Pharmacology·Lena LottigMartin Diener
Aug 29, 2012·The Journal of Pharmacology and Experimental Therapeutics·Dmitry A SibarovSergei M Antonov
May 12, 2016·American Journal of Physiology. Endocrinology and Metabolism·Sergej Pirkmajer, Alexander V Chibalin
Apr 16, 2020·American Journal of Physiology. Cell Physiology·Violetta V KravtsovaIgor I Krivoi
Oct 16, 2015·American Journal of Physiology. Cell Physiology·Palanikumar ManoharanJudith A Heiny
Nov 30, 2018·Invertebrate Neuroscience : in·Arkady S PivovarovRobert J Walker
Mar 3, 2019·International Journal of Molecular Sciences·Igor I Krivoi, Alexey M Petrov
Apr 25, 2020·International Journal of Molecular Sciences·Violetta V KravtsovaIgor I Krivoi
Oct 12, 2018·Communications Biology·Hamid R NooriSteven M Sine
Nov 17, 2019·Cell Calcium·Amir Askari
Jan 6, 2021·Journal of Muscle Research and Cell Motility·Sergej PirkmajerAlexander V Chibalin
Feb 18, 2021·American Journal of Physiology. Cell Physiology·Daiki Watanabe, Masanobu Wada
May 1, 2021·International Journal of Molecular Sciences·Violetta V KravtsovaIgor I Krivoi

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