PMID: 698285Sep 4, 1978Paper

On the interaction of histone Hl and Hl peptides with DNA. Sedimentation, thermal denaturation and solubility studies

Biochimie
F J AvilesA Albert

Abstract

The interactions in buffered 0.14 M NaCl (pH 7.0) of DNA, native or sonicated, with histone Hl and with its fragments N-Hl (residues 1-72) and C-Hl (residues 73-COOH), have been studied by using the techniques of sedimentation, thermal denaturation and solubility. Histone Hl shows a preferential affinity for high molecular weight DNA in comparison with sonicated DNA. The binding of histone Hl to sonicated DNA in 0.14 M NaCl is reversible and the reversibility decreases with time. These findings are consistent with the view that Hl is cooperatively distributed along DNA molecules and that this distribution is energetically more favoured for long DNA than for sonicated DNA. It has been found that under conditions of moderate salt concentration, the C-terminal region is the main one responsible for the interaction of Hl with DNA, and also for a cooperative distribution of the histone along the DNA molecules. Addition of urea to the solution produces a decrease of solubility of Hl-DNA complexes. This effect reflects an additional condensation of the complex, which is probably related to the unfolding of the globular part of the histone. It is suggested that this globular region does not play a substantial role in the condensation o...Continue Reading

References

Jan 25, 1977·Journal of Molecular Biology·M Noll, R D Kornberg
Jul 1, 1976·European Journal of Biochemistry·A J VarshavskyG P Georgiev
Aug 8, 1975·Nucleic Acids Research·V V BakayevA J Varshausky
Aug 14, 1969·Journal of Molecular Biology·D E Olins
Jan 25, 1974·Science·A L Olins, D E Olins
Nov 19, 1974·Biochemistry·J A D'Anna, I Isenberg
May 24, 1974·Science·R D Kornberg, J O Thomas
Feb 1, 1969·The Biochemical Journal·E W Johns, S Forrester
Nov 1, 1970·European Journal of Biochemistry·M C Touvet-PoliakowM H Champagne

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