On the interaction of neomycin with the slow vacuolar channel of Arabidopsis thaliana

The Journal of General Physiology
Joachim Scholz-StarkeF Gambale

Abstract

This study investigates the interaction of the aminoglycoside antibiotic neomycin with the slow vacuolar (SV) channel in vacuoles from Arabidopsis thaliana mesophyll cells. Patch-clamp experiments in the excised patch configuration revealed a complex pattern of neomycin effects on the channel: applied at concentrations in the submicromolar to millimolar range neomycin (a) blocked macroscopic SV currents in a voltage- and concentration-dependent manner, (b) slowed down activation and deactivation kinetics of the channel, and most interestingly, (c) at concentrations above 10 muM, neomycin shifted the SV activation threshold towards negative membrane potentials, causing a two-phasic activation at high concentrations. Single channel experiments showed that neomycin causes these macroscopic effects by combining a decrease of the single channel conductance with a concomitant increase of the channel's open probability. Our results clearly demonstrate that the SV channel can be activated at physiologically relevant tonoplast potentials in the presence of an organic effector molecule. We therefore propose the existence of a cellular equivalent regulating the activity of the SV channel in vivo.

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Citations

Oct 30, 2012·Journal of Experimental Botany·Paul Vijay Kanth GutlaArmando Carpaneto
Oct 10, 2008·Annual Review of Physiology·John M WardJulian I Schroeder
Apr 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aaron B Stephan, Julian I Schroeder
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Jan 1, 2018·Functional Plant Biology : FPB·Igor Pottosin, Oxana Dobrovinskaya
May 2, 2018·World Journal of Gastroenterology : WJG·Erika Hernández-Aquino, Pablo Muriel
Jul 13, 2017·Scientific Reports·Irene PafumiArmando Carpaneto

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