PMID: 7520694Aug 15, 1994Paper

On the interactions of Ca2+ and cyclosporin A with a mitochondrial inner membrane pore: a study using cobaltammine complex inhibitors of the Ca2+ uniporter

The Biochemical Journal
M Crompton, L Andreeva

Abstract

The mitochondrial inner membrane contains a Ca(2+)-activated pore of possible relevance to the pathogenesis of ischaemia/reperfusion injury which is inhibited by the immunosuppressant cyclosporin A (CSA). The present study employs a number of novel cobaltammine complex inhibitors of the Ca2+ uniporter (mediating Ca2+ uptake) to examine whether intramitochondrial Ca2+ influences the capacity of CSA to block the pore. Using dissipation of the inner membrane potential as a means of monitoring the state of the pore, it is shown that CSA blockade is facilitated as Ca2+ uptake is restricted. Ca2+ also depresses and reverses the binding of [3H]CSA to mitochondria, but Ca2+ is ineffective when its uptake is prevented. It is concluded that a high intramitochondrial Ca2+ concentration antagonizes pore inhibition by CSA. The significance of this is discussed.

Citations

May 15, 1996·European Journal of Biochemistry·A TanveerM Crompton
Jul 6, 2007·Pflügers Archiv : European journal of physiology·Wolfgang F GraierRoland Malli
Jul 17, 1999·European Journal of Biochemistry·N JohnsonM Crompton
Nov 18, 2000·The Journal of Physiology·M R Duchen
Dec 24, 2019·Current Opinion in Chemical Biology·Joshua J Woods, Justin J Wilson
May 23, 2002·Biochimie·Andrew P HalestrapSamantha J Clarke
Apr 25, 2000·Biochimica Et Biophysica Acta·O HermeshJ Bar-Tana
Oct 26, 1999·Bioorganic & Medicinal Chemistry·J F UnittF Ince
May 28, 2021·Chemical Communications : Chem Comm·Joshua J WoodsJustin J Wilson
Aug 11, 2004·Molecular Aspects of Medicine·Michael R Duchen
May 16, 2006·Biochimica Et Biophysica Acta·Marco Di Paola, Michele Lorusso
May 31, 2008·Biochimica Et Biophysica Acta·Martin Klingenberg

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