PMID: 6404633May 2, 1983Paper

On the interrelation between calmodulin and EGTA in the regulation of the affinity to Ca2+ and the maximal activity of the erythrocyte-membrane calcium pump

European Journal of Biochemistry
S N OrlovY V Postnov

Abstract

1. Calmodulin distribution in rat erythrocytes and the interrelation between calmodulin and EGTA in regulation of the rate of 45Ca accumulation by erythrocyte-membrane inside-out vesicles were studied. 2. The total content of calmodulin in rat erythrocytes is about 24 mumol/l of cells. About 60% of calmodulin is localized in cytoplasm and 40% (10 mumol/l of cells) of calmodulin is loosely bound to membranes; after subsequent washing by hypotonic solution (membranes A) the content of membrane-bound calmodulin decreases up to 0.1 mumol/l of cells. 3. The addition of exogenous calmodulin to membranes A results in increase of the maximal activity of the Ca-pump and does not influence its affinity to Ca2+. Troponin I (30 microM) completely abolishes the calmodulin effect on the Ca-pump activity without significant alterations in its basal activity. 4. The addition of EGTA in the membrane-washing solution results in decrease of the membrane-found pool calmodulin up to 0.01 mumol/l of cells (membranes B). This procedure is accompanied by decrease of the affinity of Ca-pump to Ca2+ and does not influence its maximal rate. 5. The effect of EGTA treatment (membranes B) on the affinity of Ca-pump to Ca is abolished after addition of micro...Continue Reading

References

Jan 8, 1976·Biochimica Et Biophysica Acta·M G LuthraD J Hanahan
May 6, 1975·Biochimica Et Biophysica Acta·S Katz, R Blostein
Jan 1, 1978·The International Journal of Biochemistry·K S Au
Apr 1, 1972·The Journal of General Physiology·M L Weiner, K S Lee
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Dec 1, 1980·Biochimica Et Biophysica Acta·M G LuthraH D Kim
Jan 1, 1980·Annual Review of Biochemistry·C B KleeP G Richman

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Citations

Nov 1, 1993·Biulleten' eksperimental'noĭ biologii i meditsiny·N V Maksimova, V V Petrov
Oct 24, 2003·Cell Calcium·Sergei N OrlovSergei V Kotelevtsev
Aug 1, 1985·European Journal of Clinical Investigation·U Walter, S Müller
Feb 27, 1986·Biochimica Et Biophysica Acta·M A Birch-Machin, A P Dawson
Oct 30, 1985·Biochemical and Biophysical Research Communications· Alakhov VYuS M Dudkin

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