PMID: 6112229Dec 1, 1980Paper

On the involvement of the Golgi apparatus and GERL in processing of secretion products and some enzyme activities in the rat pituitary mammotroph

Journal of Cell Science
A J Nevorotin

Abstract

Whole pituitary of Wistar albino rats were either (a) immediately fixed in an aldehyde fixative (Karnovsky), or incubated prior to the fixation in (b) unaltered medium 199, (c) medium 199 supplemented with 1.65 M sucrose, or (d) medium 199 diluted with distilled water (1:2). Incubation time was for 0.5 h, at a temperature of 4 degrees or 37 degrees C. All specimens were routinely processed for acid phosphatase (AcPase) or thiamine pyrophosphatase (TPPase) demonstration and examined ultrastructurally. In the control series a and b AcPase activity in the mammotroph was associated with the cisternae of GERL, dense bodies, coated vesicles, immature secretion granules often continuous with cisternae of GERL, and occasionally with the innermost lamellae of the of the Goldi apparatus (GA). TPPase activity was found within the innermost lamellae and the fenestrated element of GA. Occasional granular cores were seen within TPPase-positive lamellae. In all mammotrophs of series (4 degrees and 37 degrees C) severe shrinkage of the lamellae of GA and other membrane-bound structures in the Golgi area was apparent. In some mammotrophs of the series c (37 degrees C) AcPase activity, in addition to its usual sites, was revealed within all lame...Continue Reading

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